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1、VIRALIMMUNOLOGYVolume21,Number2,2008©MaryAnnLiebert,Inc.Pp.153–161DOI:10.1089/vim.2007.0108IdentificationofaNovelTranscriptionalRepressor(HEPIS)ThatInteractswithnsp-10ofSARSCoronavirusMINHONG,WEIZHONGLI,LICHUNWANG,LIJIANG,LONGDINGLIU,HONGLINGZHAO,andQIHANLIABS
2、TRACTAnovelgenewaspreviouslyisolatedfromacDNAlibraryofhumanembryolungtissuebyitsen-codedprotein,whichinteractswithnon-structuralprotein10(nsp-10)ofthesevereacuterespira-torysyndromecoronavirus(SARS-CoV).Theproteinwasnamedhumanembryolungcellularpro-teininteract
3、ingwithSARS-CoVnsp-10(HEPIS),anditiscomposedof147aminoacidswithseveralCKIIphosphorylationsites.Inthepresentstudy,wedemonstratedthatHEPISwascapableofsup-pressingchloramphenicolacetyltransferase(CAT)geneexpressioncontrolledbydifferentenhancerelementsinatranscrip
4、tionassay.HEPISinteractedspecificallywiththeHSP70TATAsequence,andnotwithvariousotherenhancerelementsinabindingtest.Furthermore,weco-immunopre-cipitatedHEPISwithBTF3,acomponentoftheRNApolIIinitiationcomplex,andobservedre-ducedproliferationofHeLacellstransfected
5、withtheHEPISgene.Takentogether,ourresultssug-gestthatHEPISmayfunctionasapotentialtranscriptionalrepressor.ViralImmunology2008.21:153-162.INTRODUCTIONstreamoftheTATAboxtoenhancetranscriptionalactiv-ity(18).Suchamodelalsosuggeststhatvarioustran-RESEARCHONTHEREGU
6、LATIONOFEUKARYOTICGENEscriptionalrepressorsfunctionbybindingtodifferentini-EXPRESSIONduringthepreviousdecadefocusedontiators,regulatoryfactors,orspecificDNAsequences,totheidentificationandcharacterizationofthevariousfac-slowtherateoftranscription(21).Identific
7、ationofvari-torsthatareinvolvedinthetranscriptionalprocess(5,16).ousrepressors,suchasp53,supportthismodelandindi-Despitethelackoffinedetails,amodelwasestablishedcatethattherepressor,asatranscriptionalregulator,isverytodemonstratetheprocessoftranscriptionineuka
8、ryoticimportantforeukaryoticcellproliferationandmetabolismcells(1).Inthismodel,atleasttwokindsoffactorsare(14).Duringviralreplicationinhostcells,someproteinsinvolvedintranscription
