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1、Int.J.Morphol.,28(2):549-555,2010.DetectionofCoronavirusinCapybaras(Hydrochoerishydrochaeris)byTransmissionElectronMicroscopyinSãoPaulo,BrazilDeteccióndeCoronavirusinCapybaras(Hydrochoerishydrochaeris)porMicroscopíaElectrónicadeTransmisiónenSãoPaulo,Brasil*C
2、atroxo,M.H.B.;**Miranda,L.B.;***Lavorenti,A.;Petrella,S.;*Melo,N.A.;*Martins,A.M.C.P.R.F.CATROXO,M.H.B.;MIRANDA,L.B.;LAVORENTI,A.;PETRELLA,S.;MELO,N.A.;MARTINS,A.M.C.P.R.F.Detectionofcoronavirusincapybaras(Hydrochoerishydrochaeris)bytransmissionelectronmicro
3、scopy,inSãoPaulo,Brazil.Int.J.Morphol.,28(2):549-555,2010.SUMMARY:Coronavirusesareenveloped,positive-strandedRNA,belongstotheNidoviralesorder,Coronaviridaefamilyandareclassifiedintothreegroups.Groups3aand3ccomprisingtheaviancoronaviruses.Theyareworldwideindi
4、stributionandcancauserespiratory,entericandinsomecaseshepatitisandneurologicaldiseasesinawidevarietyofanimals,resultingineconomiclossestobreedersandbreeding.Inthisstudy,wedescribeanoutbreakofdiarrheainaherdofcapybarasfoundinthecityofPiracicaba,SãoPaulo,Brazi
5、l.Theanimalshadsuddensymptomsofanorexia,profuseandwaterydiarrhea,yellowishorgreenish,dehydration,weightloss,sharpandtwistingoflimbsleadingtodeathinlessthanaweek,mostlyweanedandwithanageofthreemonths.Atotalof59stoolsamplesand10fragmentsofsmallintestinewerepro
6、cessedfornegativestainingtechnique(rapidpreparation).Bytransmissionelectronmicroscopealargenumberofparticleswithmorphologysimilartocoronavirus,pleomorphic,roundedorelongatedfeatureswithradialprojectionsformacoronaandmeasuring80-140nmindiameter,wasvisualizedi
7、nallsamplesanalyzed.Thetechniquewasextremelyusefulforrapidviraldiagnosisintheaffectedanimals.KEYWORDS:Coronavirus;Capybaras;Transmissionelectronmicroscopy.INTRODUCTIONCoronavirusesareworldwideindistribution,highlyTheentericcoronavirusesischaracterizedbyano-i
8、nfectious,andextremelydifficulttocontrol.Theycancau-rexia,prostration,dehydration,dyspneaanddeathofanimalsserespiratory,entericandinsomecaseshepaticandofallages,mainlyyoungandthosesubmittedtoane
