冠状病毒Coronavirusl论文-2005 Susceptibility of different eukaryotic cell lines to SARS-coronavirus.pdf

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1、ArchVirol(2005)150:10231031DOI10.1007/s00705-004-0461-1SusceptibilityofdifferenteukaryoticcelllinestoSARS-coronavirusBriefReportK.Hattermann∗,M.A.M¨uller∗,A.Nitsche,S.Wendt,O.DonosoMantke,andM.NiedrigRobertKoch-Institut,CenterforBiologicalSafety1,Berlin,GermanyReceivedSeptember16,2004;

2、acceptedNovember1,2004PublishedonlineJanuary13,2005cSpringer-Verlag2005Summary.InordertodefineandcharacterizetargetcellsofSARS-coronavirus(SARS-CoV)westudiedthesusceptibilityof23differentpermanentandprimaryeukaryoticcelllinestoSARS-coronavirus.BeneathVeroE6cellsSARS-Coronavirusinfectio

3、ncouldalsobedemonstratedintwopigcelllines(POEK,PS)andonehumancellline(Huh-7)usingtheindirectimmunofluorescenceassayandanewlyestablishedquantitativereal-timePCR.InallsusceptiblecelllinesmRNAoftheAngiotensin-convertingenzyme2(ACE2),thefunctionalreceptorforSARS-CoVinfection,couldbedetected

4、byRT-PCR.OurresultsshowthatthereisacorrelationbetweentheabundanceofACE2mRNAandSARS-CoVsusceptibility.∗Anovelcoronaviruswithapositivesingle-strandedRNAgenomeofapprox-imately30.000bphasbeenidentifiedasthecausativeagentofsevereacuterespiratorysyndrome(SARS)[3,8].TheSARS-coronavirus(SARS-Co

5、V)infectsthelowerrespiratorytractwithpatientspresentingfever,drycough,dyspnoea,headacheandhypoxemia[7].ThediseasespreadrapidlyfromSouthernChinatomorethan30countrieswithinafewweeksresultinginabout8.400casesand800deaths[13].ThefunctionalcellularreceptorforSARS-CoVcouldrecentlybeidentified

6、astheAngiotensin-convertingenzyme2(ACE2)[9].SofarthereservoirofSARS-CoVhasnotyetbeenpreciselyclarified.Theviruscouldbeisolatedoutofpalmcivets(Pagumalarvata),raccoondogs(Nycereutesprocyonoides)andothermammalsthatareforsaleonliveanimal∗Theseauthorscontributedequallytothisarticle.1024K.Hat

7、termannetal.marketsandsometimeseateninChina[2].However,thesespeciesarenotneces-sarilythenaturalhosts.InourstudyweinvestigatedifSARS-CoVcouldinfectandreplicateinpermanentcelllinesandprimarycellsofdifferentspeciesinordertoi)defineandcharacterizepotentialtargetcellsofSARS-CoV,ii)tounders