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1、JournalofMedicalVirology5:221-229(1980)IsolationofRhinovirusesandCoronavirusesFrom38ColdsinAdultsH.E.Larson,SylviaE.Reed,andD.A.J.TyrrellMedicalResearchCouncilClinicalResearchCentre,WatfordRoad,Harrow,Middlesex(H.E.L.,D.A.J.XI,andtheCommonColdUnit,Salisbury,Wiltshire(S.E.R.,D.A.J.XINasalwas
2、hingswerecollectedfrom27normaladultsduring38naturallyacquiredcolds.Thewashingswereexhaustivelytestedusingtissuecultures,organculturesandelectronmicroscopy.Washingsyieldingnoidentifiableagentwereinoculatedintohumanvolunteers,andfurtherspecimensob-tainedfromthelatterwereexaminedbythesametechn
3、iquesinvitro.Viruseswereidentifiedinassociationwith25oftheoriginal38colds(65.7%).Fif-teenwererhinoviruses(39.5%),sevencoronaviruses(18.4%),twowerepara-influenzaviruses,andonewasinfluenzavirus.Useoforganculturesandofvolunteerssignificantlyincreasedtheisolationrate.Noagentwascultivatedfromthe
4、remaining13specimens,althoughtestsinVolunteersshowedthatcold-producingagentswerepresentinfiveofthem(13%).Threespecimensgavedoubtfulresultsinvolunteers,andfiveothers,allcollectedwithinaperiodofsixweeksinDecemberandJanuary,apparentlycontainednoinfectiousagent.Keywords:commoncold,rhinovirus,co
5、ronavirus,volunteers,organcultureINTRODUCTIONAlthoughavarietyofvirusesareknowntocauserespiratoryinfections,inclinicalpractice,ratesofvirusisolationfromtherespiratorytractremainobstinatelylowandnormallydonotexceed30%-40%ofspecimenstested[Higgins,19741.Isolationofmanyoftheagentsresponsible,su
6、chascoronaviruses,somerhinoviruses,andsomeparainflu-enzavirusesisknowntopresenttechnicaldifficulties,andtheuseofhumanembryonicnasalortrachealorganculture,althoughlaborious,hasbeenshowntoincreaseisolationrates[TyrrellandBynoe,1966;HigginsandEllis,19731.Ithasremaineduncertainwhetheravailablet
7、echniques,includingorgancultureandelectronmicroscopy,arecapableofidentifyingthelargemajorityofupperrespiratoryinfections,orwhetherasignificantproportionofsuchinfectionsisassociatedwithagentsnotcultivableevenbytheseelaboratemethods.Thepresentstudywasunder
