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1、MicrobialPathogenesis1986,l:573582RegulationofviralpersistenceinhumanglioblastomaandrhabdomyosarcomacellsinfectedwithcoronavirusOC43ArleneR.Collins’andOleSorensen’’VirusLaboratory,DepartmentofMicrobiology,StateUniversityofNewYorkatBuffalo,Buffalo,NewYork14214,U.S.A.and2Cyto
2、biologyGroup,DepartmentofMicrobiologyandimmunology,UniversityofWesternOntario,London,Ontario,CanadaN6A6Cl(ReceivedMarch11,1986;acceptedinrevisedformJune16,1986)Collins,A.R.(DeptofMicrobiology,StateUniversityofNewYork,Buffalo,NY14214,U.S.A.)and0.Sorensen.Regulationofviralper
3、sistenceinhumanglioblastomaandrhabdomyosarcomacellsinfectedwithcoronavirusOC43.MicrobialPathogenesis,1986;1:573-582.Culturesofhumanrhabdomyosarcoma(RD)andhumanglioblastoma(U87-MG)werecomparedfortheirabilitytosustainapersistentinfectionwithcoronavirusOC43.Within28days,infect
4、iousvirusandhemagglutininwerebeingproducedathighlevelsinbothtypesofcells.Temperaturesensitiveplaquevariantswererecoveredat31°C.Inbothcelltypes,theviruscausedincreasedantigensynthesisandcelldeath,ifthetemperaturewasloweredto31°C.Infectiousviruswaslostifcellsweretreatedwithan
5、tiserumtowholevirusorifthetemperaturewasraisedto39.5”C.ProbingthecuredcellswithOC43-specific32P-cDNAshowedthatcuredcellscontainednodetectableviralRNA.Therelativeeaseofestablishmentandcureofthesepersistentinfectiousmakesthemattractiveasmodelstostudycoronavirusregulatoryproce
6、sses.Keywords:coronaviruses;persistence;cDNAprobes;neurotropism.IntroductionOC43isahumanrespiratoryvirusofthefamilyCoronaviridae.Itwasoriginallyisolatedinhumantrachealorgancultures’andthenadaptedtogrowinsucklingmice.Althoughclinicalisolationfrompatientmaterialrequirestheuse
7、oforgancultures,anumberofhumancelllinescanbeproductivelyinfectedwithOC43virusandaplaqueassayhasbeendeveloped.*Ininfectedmiceage-relatedsusceptibilitytothevirusisdisplayedandthereisaselectivevulnerabilityofneuronalcells.3Infectedmouseneuronalcellsinculturedisplaydifferential
8、susceptibility,acharacteristicfeatureforallcoronavirusinfections.Neuronsderivedfro
