血清铁调素在骨质疏松模型中变化趋势的观察.doc

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1、血清铁调素在骨质疏松模型中变化趋势的观察【摘要】目的研究SD大鼠维甲酸灌胃骨质疏松模型中血清hepcidin含量的变化。方法取72只大鼠分为模型组和对照组，模型组以维甲酸70mg/(kg•d)持续灌胃14天，对照组等量蒸憾水灌胃14天，双能X线骨密度仪器进行活体骨密度测定，模型组和对照组血清hepcidin含量EIJSA试剂盒进行测定。结果骨密度的测定显示骨质疏松模型组骨密度较对照组降低，差异有显著性。模型组和对照组相比较，血清hepcidin含量在模型建立过程中有变化:初期略有降低,差异无显著性；中期明显降低，差异有显著

2、性；后期略有回升，低于对照组，差异无显著性。结论血清hepcidin含量变化与维甲酸致骨质疏松形成可能存在直接或间接关系，hepcidin的变化可能成为骨质矿化异常诊断、治疗的新焦点，为进一步研究骨质疏松提供新的思路。【关键词】骨质疏松维甲酸铁调素血清ObservationofchangesofseroushepcidinconcentrationinSDratsosteoporosismodel[Abstract]ObjectiveToinvestigatethechangesofserumhepcidinconcent

3、rationinSDratsosteoporosismodelinducedbyintragasticadministrationwithtretinoin.Methods72SI)ratsweredividedintoexperimentalgroupandcontrolgrouprandomly,treatedtheexperimentalgroupwithtretinoinandthecontrolgroupwithdistilledwaterofequalquantitybyintragasticadminista

4、tionforlasted14days,assayedthebonedensitywithdualenergyX-rayabsorptiometry(DEXA)andserumhepcidinconcentrationofexperimentalgroupandcontrolgroupwithenzyme-linkedimmunosorbentassay(ELISA)・ResultsTheassayofbonedensityindicatedthatbonedensityofexperimentalgroupwasmuch

5、lowerthancontrolgroup・Incontrasttocontrolgroup,serumhepcidinconcentrationofexperimentalgroupchangedintheformationprocessofosteoporosismode1.Theserumhepcidinconcentrationdecreasedslightlywithnosignificantdifferenceatearlystage,decreasedobviouslywithsignificantdiffe

6、reneeatintermediatestage・Butincreasedslightlyatlatestage,sti111owerthanconcentrationofserumhepcidinofcontrolgroup,decreasewasofnostatisticsmeaning・ConclusionThechangesofserumhepcidinconcentrationmayhavedirectorindirectrelationwithosteoporosisformationinducedbyintr

7、agasticadministrationwithtretinoin.Perhapshepcidininvestigationwi11becomeanewfocuspointindiagnosisandtreatmentofbonemineralizationabnormalityandmakegreatprogressinstudyingosteoporosis.[Keywords]osteoporosis；tretinoin；hepcidin；serum近几年来，许多研究结果认为体内铁代谢平衡和紊乱与骨质矿化有着密切的

8、联系。在研究绝经女性骨质疏松治疗、预防时发现铁的摄入与骨的矿化密度呈正相关［l,2］oDenisM.Medeiros等研究发现铁缺乏饮食的骨矿化不良［3］oMargaretM.Harris等［4］研究结果显示绝经后妇女的饮食铁摄入增加可以提高骨密度。铁调素（hepcidin）是一种富含半胱氨酸的抗菌多肽