欢迎来到天天文库
浏览记录
ID:50524515
大小:83.00 KB
页数:10页
时间:2020-03-10
《血清铁调素在骨质疏松模型中变化趋势的观察.doc》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、血清铁调素在骨质疏松模型中变化趋势的观察【摘要】目的研究SD大鼠维甲酸灌胃骨质疏松模型中血清hepcidin含量的变化。方法取72只大鼠分为模型组和对照组,模型组以维甲酸70mg/(kg•d)持续灌胃14天,对照组等量蒸憾水灌胃14天,双能X线骨密度仪器进行活体骨密度测定,模型组和对照组血清hepcidin含量EIJSA试剂盒进行测定。结果骨密度的测定显示骨质疏松模型组骨密度较对照组降低,差异有显著性。模型组和对照组相比较,血清hepcidin含量在模型建立过程中有变化:初期略有降低,差异无显著性;中期明显降低,差异有显著
2、性;后期略有回升,低于对照组,差异无显著性。结论血清hepcidin含量变化与维甲酸致骨质疏松形成可能存在直接或间接关系,hepcidin的变化可能成为骨质矿化异常诊断、治疗的新焦点,为进一步研究骨质疏松提供新的思路。【关键词】骨质疏松维甲酸铁调素血清ObservationofchangesofseroushepcidinconcentrationinSDratsosteoporosismodel[Abstract]ObjectiveToinvestigatethechangesofserumhepcidinconcent
3、rationinSDratsosteoporosismodelinducedbyintragasticadministrationwithtretinoin.Methods72SI)ratsweredividedintoexperimentalgroupandcontrolgrouprandomly,treatedtheexperimentalgroupwithtretinoinandthecontrolgroupwithdistilledwaterofequalquantitybyintragasticadminista
4、tionforlasted14days,assayedthebonedensitywithdualenergyX-rayabsorptiometry(DEXA)andserumhepcidinconcentrationofexperimentalgroupandcontrolgroupwithenzyme-linkedimmunosorbentassay(ELISA)・ResultsTheassayofbonedensityindicatedthatbonedensityofexperimentalgroupwasmuch
5、lowerthancontrolgroup・Incontrasttocontrolgroup,serumhepcidinconcentrationofexperimentalgroupchangedintheformationprocessofosteoporosismode1.Theserumhepcidinconcentrationdecreasedslightlywithnosignificantdifferenceatearlystage,decreasedobviouslywithsignificantdiffe
6、reneeatintermediatestage・Butincreasedslightlyatlatestage,sti111owerthanconcentrationofserumhepcidinofcontrolgroup,decreasewasofnostatisticsmeaning・ConclusionThechangesofserumhepcidinconcentrationmayhavedirectorindirectrelationwithosteoporosisformationinducedbyintr
7、agasticadministrationwithtretinoin.Perhapshepcidininvestigationwi11becomeanewfocuspointindiagnosisandtreatmentofbonemineralizationabnormalityandmakegreatprogressinstudyingosteoporosis.[Keywords]osteoporosis;tretinoin;hepcidin;serum近几年来,许多研究结果认为体内铁代谢平衡和紊乱与骨质矿化有着密切的
8、联系。在研究绝经女性骨质疏松治疗、预防时发现铁的摄入与骨的矿化密度呈正相关[l,2]oDenisM.Medeiros等研究发现铁缺乏饮食的骨矿化不良[3]oMargaretM.Harris等[4]研究结果显示绝经后妇女的饮食铁摄入增加可以提高骨密度。铁调素(hepcidin)是一种富含半胱氨酸的抗菌多肽
此文档下载收益归作者所有