内质网应激在小鼠狼疮肾炎中的发病机制研究.pdf

《内质网应激在小鼠狼疮肾炎中的发病机制研究.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、201420790内质网应激在小鼠狼疮肾炎中的发病机制研究韦颖导师姓名：谭鹤长专业名称：内科学申请学位类型：专业学位答辩委员会主席：王明军答辩委员会委员：伍巧源黄向阳孟晓燕罗春明二○一七年五月1198901/2007.092012.062012.072013.102014.09/2014.12目录...................................................................................................1ABSTRACT........................

2、................................................................6..........................12............................................................................................12..................................................................................15..........

3、..................................................................................30............................................................................................44............................................................................................50.........

4、..................................................................................51.................................................................................................58.................................................................................................6

5、0.................................................................................................702017内质网应激在小鼠狼疮肾炎中的发病机制研究摘要systemiclupuserythematosus,SLEimmunecomplexesIClupusnephritis,LN[1]LNLNendoplasmicreticulumstress,ERSLNMRL/lpr4-4-phenylbutyricacid,4-PBAERSLNLN112MRL/lpr1

6、64-PBA88124-PBA1.0g.kg-1.d-14-PBA48122412SCrBUN120172HEPAS3CD3+T4RT-PCRmRNARNAGAPDHGRP78CHOPPERKeIF2αATF4IRE1αXBP1ATF6mRNA5WesternblotGAPDHGRP78CHOPp-PERKp-eIF2αATF4p-IRE1αXBP1ATF6FibrillarinNF-κBp656ELISAELISAMCP-1TNF-αIL-67(ANOVA)LSD-tp<0.05220171MRL/lprLNERSMRL/lprERSGRP78mRNAG

7、RP78p<0.05ERSPERKIRE1αp<0.052MRL/lprMRL/lpr1224p<0.054-PBA124-PBA24p<0.053MRL/lprBUNSCrp<0.054-PBA124-PBABUNSCrp<0.054MRL/lprp<0.054-PBA124-PBAp<0.055MRL/lprCD3+Tp<0.054-PBA124-PBACD3+T32017p<0.056MRL/lprGRP78CHOPPERKeIF2αATF4IRE1αXBP1ATF6mRNART-PCRGRP78CHOPPERKeIF2αATF4IRE1αXBP1A

8、TF6mRNAp<0.054-PBA124-PBAp<0.057M