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1、参附注射液对大鼠短暂性局灶性脑缺血损伤的治疗时间窗作者：郑玉，熊利泽，朱正华，王强，陈绍洋【关键词】参附注射液关键词：参附注射液;脑缺血;治疗时间窗摘要:目的探讨参附注射液对局灶性脑缺血性损伤的治疗时间窗.方法80只雄性SD大鼠，随机分为8组侮组n=10):对照组,即单纯缺血再灌注组;参附注射液治疗A〜G组，分别于缺血再灌注后0,0.5,1.0,1.5,2.0,4.0和6.0h经腹腔注射参附注射液，剂量为10mLkg-1.用右侧颈内动脉线栓法致大脑中动脉阻闭120min,拔出尼龙线即刻为再灌注时间•观察再灌注后24h时神经功能损害改变并评分，24h时处死动物，取大脑行TTC染色
2、以测量脑梗死容积.结果24h时神经功能损害评分，A-F组分别为:0.5±0.3,0.5±0.4,0.6±0.5,0.8±0.4,0.8±0.3和0.9±0.3,明显低于对照组(2・1±0・8)及G组(1・5±0・7)(P<0.05)•脑梗死容积A-F组分别为(46・4±26・1),(60.2±25.8),(67.2±36.7),(76.0±39.7),(94.1±34.9)和(98.4±35・3)mm3;均明显小于对照组(182.6±39.6)mm3及G组(139・1±42.8)mm3(P<0.05),A~F组间24h时神经功能损害评分和梗死容积无差别(P>0
3、・05)・结论参附注射液对局灶性脑缺血再灌注损伤的最佳治疗时机在缺血后4h以内.Keywords:Shenfusolution;cerebralischemia;therapeutictimewindowAbstract:AIMToinvestigatethetherapeutictimewindowforShenfusolutionintransientfocalcerebralischemicinjuryinrats.METHODS80maleSDratsweighing280~350gwererandomizedinto8groups(n=10ineachgroup):C
4、on-trolgroup,receivingnopharmacologicalintervention,GroupsA〜G,receivingintraperitonealinjectionofShenfusolution(lOmLkg-1)atO,0.5,1.0,1.5,2.0,4.0and6.0hrespectivelyaiterl20minmiddlecerebralarteryocclu-sion(MCAO)with3-0nylonmonofilamentviatherightin-ternalcarotidartery.Theneurologicaloutcomesw
5、ereevalu-ated24hafterthereperfusion.ThebraininfarctvolumeswerethenassessedbyTTCstaining.RESULTSTheneuro-logicaldeficitscores(NDS)forGroupsA〜F24hafterthereperfusionwere0.5±0.3,0.5±0.4,0.6±0.5,0.8±0.4,0・8±0.3and0・9±0・3,respectively,whichwereobviouslylowerthanthoseofthecontrolgroup(2.1土0.8)andG
6、roupG(1.5±0.7)(P<0.05).Theinfarctvolumesofthecontrolgroup(182.6±39.6)mm3andGroupG(139.1±42.8)mm3weresignificantlylargerthanthoseofGroupsA~F(46.4±26.1),(60.2±25.8),(67.2±36.7),(76.0±39.7),(94.1±34.9)and(98.4±35.3)mm3,respectively(P<0.05)24hafterthereperfusion.Therewerenodifferencesbetwe
7、enGroupsA~FinbothNDSandinfarctvolume(P>0.05)・CONCLUSIONTherapeutictimewindowforShenfusolu-tionintransientfocalcerebralischemicinjurylastsforatleast4h・0引言急性脑缺血/再灌注损伤可引起严重的神经功能障碍，甚至危及生命•如何防治脑缺血/再灌注损伤，寻找有效的治疗药物及措施,以促进脑卒中后中枢神经系统功能恢复一直是相关学科研究的重点之一•近年
