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时间:2020-02-29
《热凝胶多糖产生菌株的复合诱变选育 优先出版.pdf》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、DOI:10.13989/j.cnki.0517-6611.2017.11.003网络出版时间:2017-05-0408:33:28ST网络出版地址:UVWX,Journalhttp://kns.cnki.net/kcms/detail/34.1076.S.20170504.0833.006.htmlofAnhuiAgri.Sci.2017,45(11):7-10³´µ¶·¸%#`¹º»¼¯12231=>?,@!,AA,!BC,D"E(1.#1%&ø&øJ'a&*,5E201620;2.5E#(Ä)º_)&,5E200333;3.1#*+%&'()&&*,5
2、E200241)12[-.]!FGHIJopLGHü×。[AB]KgÇLMLNO[PSQR%3714xÐST;ùUV-bW;3、1.57×10L1.27×10Da。[s]guST!vFGHIJ.opXvwvIJ.GHü×。345FGHIJ;ÇLST;NO[PST;!o3Ë;GHü×6789:Q93;<=>?A;@A:0517-6611(2017)11-0007-04MutationandScreeningofCurdlanproducingStrainbyCombinedMutationMethods1221FUYaxin,LEIGao,FANGQing,YANGXuexiaetal(1.CollegeofChemistry,ChemicalEngineeringandBio4、technology,DonghuaUniversity,Shanghai201620;2.ShanghaiDongshengFoodCo.Ltd,Shanghai200333)Abstract[Objective]Inordertoimprovetheyieldandqualityofcurdlan.[Method]Agrobacteriumsp.714wasmutatedbyUVandNTG.Twohighyieldstrainswereselectedbyplatespreadingandshakingflaskfermentation.[Resul5、t]Thecrudeyieldofcurdlanfromthestrain0.6-45andstrain0.5-50were26.4and26.5g/L,andwere6.9and7.0timeshigherthanthecrudeyieldofparentstrain(3.8g/L).Therefinedyieldofcurdlanfromthestrain0.6-45andstrain0.5-50were19.4and18.0g/L.Thecharacteristicsofgelshowedthatthefracturestrength,fractured6、istance,springiness,cohesivenessandresiliencewerehigherthanthesamplefrommarket,andthegelstrength,hardness,gumminessand66chewinesswerelowerthansamplefrommarket.GPCresultsshowedthemolecularweightswererespectively1.57×10and1.27×10Daforcurdlanfromthestrain0.6-45and0.5-50.[Conclusion]Com7、binedmutationimprovestheyieldofcurdlanandchangesthecharacteristicsofgel.KeywordsCurdlan;UVmutation;NTGmutation;Highyieldstrain;Gelcharacteristics,¢H+-.Ð,Oúo/0+xt%'SßTqU。,¢H+XÝÄ¢Ív¤,½[1-3]'aó'2Òβ-1,3-)ò+,,ôõnÇqú±2ó^Vqú»2¢{Å,áÝW,ÑÒ[4]¡}Ý¢21{Åb¼«。,¢H+Z2Ó,¢H+2×ØOú:h9±ó^,¼wx¯þ,¢[5]19668、.0HaradaJ4U9、½º×Ø,19
3、1.57×10L1.27×10Da。[s]guST!vFGHIJ.opXvwvIJ.GHü×。345FGHIJ;ÇLST;NO[PST;!o3Ë;GHü×6789:Q93;<=>?A;@A:0517-6611(2017)11-0007-04MutationandScreeningofCurdlanproducingStrainbyCombinedMutationMethods1221FUYaxin,LEIGao,FANGQing,YANGXuexiaetal(1.CollegeofChemistry,ChemicalEngineeringandBio
4、technology,DonghuaUniversity,Shanghai201620;2.ShanghaiDongshengFoodCo.Ltd,Shanghai200333)Abstract[Objective]Inordertoimprovetheyieldandqualityofcurdlan.[Method]Agrobacteriumsp.714wasmutatedbyUVandNTG.Twohighyieldstrainswereselectedbyplatespreadingandshakingflaskfermentation.[Resul
5、t]Thecrudeyieldofcurdlanfromthestrain0.6-45andstrain0.5-50were26.4and26.5g/L,andwere6.9and7.0timeshigherthanthecrudeyieldofparentstrain(3.8g/L).Therefinedyieldofcurdlanfromthestrain0.6-45andstrain0.5-50were19.4and18.0g/L.Thecharacteristicsofgelshowedthatthefracturestrength,fractured
6、istance,springiness,cohesivenessandresiliencewerehigherthanthesamplefrommarket,andthegelstrength,hardness,gumminessand66chewinesswerelowerthansamplefrommarket.GPCresultsshowedthemolecularweightswererespectively1.57×10and1.27×10Daforcurdlanfromthestrain0.6-45and0.5-50.[Conclusion]Com
7、binedmutationimprovestheyieldofcurdlanandchangesthecharacteristicsofgel.KeywordsCurdlan;UVmutation;NTGmutation;Highyieldstrain;Gelcharacteristics,¢H+-.Ð,Oúo/0+xt%'SßTqU。,¢H+XÝÄ¢Ív¤,½[1-3]'aó'2Òβ-1,3-)ò+,,ôõnÇqú±2ó^Vqú»2¢{Å,áÝW,ÑÒ[4]¡}Ý¢21{Åb¼«。,¢H+Z2Ó,¢H+2×ØOú:h9±ó^,¼wx¯þ,¢[5]1966
8、.0HaradaJ4U
9、½º×Ø,19
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