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时间:2020-02-29
《河南省猪等孢球虫的分子变异规律研究 优先出版.pdf》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、DOI:10.13989/j.cnki.0517-6611.2017.11.029网络出版时间:2017-05-0408:34:59ab网络出版地址:cdeT,Journalhttp://kns.cnki.net/kcms/detail/34.1076.S.20170504.0834.058.htmlofAnhuiAgri.Sci.2017,45(11):93-95OPQR«STU-0V~WXY123Ò,,6ß(1.ÊÕax³{Ý'öInÝ,Ê"450008;2.,Öax³{Ý'öInÝ,Ê,46
2、1000;3.Ês¥?çD¶J¦ç+,Ê"450002)23['(]¸¹L()¢½ÅÆ。[DE]LK、、、¾/S½(m)ÅL2hÌS-,bc()02;)0y(8)0/ÈI`·bcX,·rÎy¾PWghMÎybcm。[mU]LéZS½(8âg,·öu~。I.suisPW7ÏM~d,éeZ[@Á,Z[ÏIÁÓÊ(éZÏÀMÝ(ZÒç。[ms]RmUqg~¡ûü¢£Èɤ¥§4。456;2;r;Îy,7089S858.28:;<=>A:?@90517-6611(2017)11-0093-03Studyo
3、ntheMolecularVariationLawsofIsosporasuisinHenanProvince123LIUGuanghui,CHENGJunzhen,NINGChangshen(1.HenanCentreforAnimalDiseasesControlandPrevention,Zhengzhou,Henan450008;2.CenterforAnimalDiseasesControlandPreventioninXuchangCity,Xuchang,Henan461000
4、;3.CollegeofAnimalScienceandVeterinaryMedicine,HenanAgriculturalUniversity,Zhengzhou,Henan450002)Abstract[Objective]TomasterthemolecularvariationlawsofIsosporasuisinHenanProvince.[Method]Thepigmanuresampleswerecollectedfromsomelargescalepigfarmsineast
5、ern,western,southern,northernandcentralregionsofHenanProvincetoisolateandpurifyporcinecoccidianoocysts.8isolatedstrainsofswinecoccidiosiswereusedtoextractnucleicacidandsequence.ThegenesequenceofI.suiswerecomparedwiththatofotherrelatedprotozoon.[Result]
6、8swinecoccidianstrainsfromdifferentregionsofHenanProvincewereidentifiedasI.suisandtherewasnoobviousgeneticdifferences.I.suishadgreaterdifferenceswithother7kindsofprotozoon.I.suisandother7kindsofprotozoonbelongedtodifferentbiotypes.Thehomologyofdifferen
7、tkindsofprotozooninfectedinthesamekindsofanimalswaslower.[Conclusion]Theresearchresultscanprovidescientificbasisforeffectivepreventionandcontrolofpigletcoccidiosis.KeywordsIsosporasuis;Purification;Amplification;Genesequencem]²,/!ê{/¶çMá^cd,1.2LM[1-2]Ó
8、/!ê{M56{7/3ê,561.2.1Ù>ö。öäVùb,ö1~30ßéN8~15ßf,rßkê,{åkX。éMùÙ;ªú÷M5]ùS50~100g,FG"Ñò¾_8{ú÷agaùS,°`ôùSþ;8,àn,u£ç®、«0ªM],8@4℃÷w,{åM:,ùSdLV8,Íé÷(/ÙT3;¬。/_,*øÓ,h6,¥4y,eÌlm5â1.2.2¤¥±。d@E-G6UV%¬b±ê¤[3-5]ÞD4:,úôµ¶
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