ABI7500相对定量实验_PN4347966A[1].pdf

ABI7500相对定量实验_PN4347966A[1].pdf

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1、ܹ䮼ᣛफㅔҟ੠Ⳍᇍᅮ䞣Ⳍᇍᅮ䞣ᅲ偠⼎՟ᅲ偠㕢೑ᑨ⫼⫳⠽㋏㒳݀ৌ7300/7500ᅲᯊᅮ䞣PCRҾ䆒䅵Ⳍᇍᅮ䞣ᅲ偠PrimerExtendedonmRNA5′3′ᠻ㸠ড䕀ᔩ5′cDNAReversePrimerOligod(T)orrandomhexamerSynthesisof1stcDNAstrand3′5′cDNAҢⳌᇍᅮ䞣ডᑨᵓ⫳៤PCR᭄᥂೼Ⳍᇍᅮ䞣ⷨおЁ⫳៤PCR᭄᥂©Copyright2004,AppliedBiosystems.Allrightsreserved.ForResearchUseOnly.Notforuseindiagnosticpr

2、ocedures.AuthorizedThermalCyclerThisinstrument,SerialNo___________,isanAuthorizedThermalCycler.Itspurchasepriceincludestheup-frontfeecomponentofalicenseunderUnitedStatesPatentNos.4,683,195,4,683,202and4,965,188,ownedbyRocheMolecularSystems,Inc.,andundercorrespondingclaimsinpatentsou

3、tsidetheUnitedStates,ownedbyF.Hoffmann-LaRocheLtd,coveringthePolymeraseChainReaction("PCR")processtopracticethePCRprocessforinternalresearchanddevelopmentusingthisinstrument.TherunningroyaltycomponentofthatlicensemaybepurchasedfromAppliedBiosystemsorobtainedbypurchasingAuthorizedRea

4、gents.ThisinstrumentisalsoanAuthorizedThermalCyclerforusewithapplicationslicensesavailablefromAppliedBiosystems.ItsusewithAuthorizedReagentsalsoprovidesalimitedPCRlicenseinaccordancewiththelabelrightsaccompanyingsuchreagents.Purchaseofthisproductdoesnotitselfconveytothepurchaseracom

5、pletelicenseorrighttoperformthePCRprocess.FurtherinformationonpurchasinglicensestopracticethePCRprocessmaybeobtainedbycontactingtheDirectorofLicensingatAppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404.DISCLAIMEROFLICENSE:Norightsforanyapplication,includinganyinvitr

6、odiagnosticapplication,areconveyedexpressly,byimplicationorbyestoppelunderanypatentorpatentapplicationsclaiminghomogeneousorreal-timedetectionmethods,includingpatentscoveringsuchmethodsusedinconjunctionwiththePCRprocessorotheramplificationprocesses.The5'nucleasedetectionassayandcert

7、ainotherhomogeneousorreal-timeamplificationanddetectionmethodsarecoveredbyUnitedStatesPatentNos.5,210,015,5,487,972,5,804,375and5,994,056,ownedbyRocheMolecularSystems,Inc.;bycorrespondingpatentsandpatentapplicationsoutsidetheUnitedStates,ownedbyF.Hoffmann-LaRocheLtd;andbyUnitedState

8、sPatentNos.5,538,84

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