资源描述：
《ABI 73007500 实时定量PCR仪相对定量实验入门指南.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、入门指南简介和相对定量相对定量实验示例实验美国应用生物系统公司7300/7500实时定量PCR仪设计相对定量实验PrimerExtendedonmRNA5′3′执行反转录5′cDNAReversePrimerOligod(T)orrandomhexamerSynthesisof1stcDNAstrand3′5′cDNA从相对定量反应板生成PCR数据在相对定量研究中生成PCR数据©Copyright2004,AppliedBiosystems.Allrightsreserved.ForResearchUseOnly.Notforu
2、seindiagnosticprocedures.AuthorizedThermalCyclerThisinstrument,SerialNo___________,isanAuthorizedThermalCycler.Itspurchasepriceincludestheup-frontfeecomponentofalicenseunderUnitedStatesPatentNos.4,683,195,4,683,202and4,965,188,ownedbyRocheMolecularSystems,Inc.,andund
3、ercorrespondingclaimsinpatentsoutsidetheUnitedStates,ownedbyF.Hoffmann-LaRocheLtd,coveringthePolymeraseChainReaction("PCR")processtopracticethePCRprocessforinternalresearchanddevelopmentusingthisinstrument.TherunningroyaltycomponentofthatlicensemaybepurchasedfromAppl
4、iedBiosystemsorobtainedbypurchasingAuthorizedReagents.ThisinstrumentisalsoanAuthorizedThermalCyclerforusewithapplicationslicensesavailablefromAppliedBiosystems.ItsusewithAuthorizedReagentsalsoprovidesalimitedPCRlicenseinaccordancewiththelabelrightsaccompanyingsuchrea
5、gents.PurchaseofthisproductdoesnotitselfconveytothepurchaseracompletelicenseorrighttoperformthePCRprocess.FurtherinformationonpurchasinglicensestopracticethePCRprocessmaybeobtainedbycontactingtheDirectorofLicensingatAppliedBiosystems,850LincolnCentreDrive,FosterCity,
6、California94404.DISCLAIMEROFLICENSE:Norightsforanyapplication,includinganyinvitrodiagnosticapplication,areconveyedexpressly,byimplicationorbyestoppelunderanypatentorpatentapplicationsclaiminghomogeneousorreal-timedetectionmethods,includingpatentscoveringsuchmethodsus
7、edinconjunctionwiththePCRprocessorotheramplificationprocesses.The5'nucleasedetectionassayandcertainotherhomogeneousorreal-timeamplificationanddetectionmethodsarecoveredbyUnitedStatesPatentNos.5,210,015,5,487,972,5,804,375and5,994,056,ownedbyRocheMolecularSystems,Inc.
8、;bycorrespondingpatentsandpatentapplicationsoutsidetheUnitedStates,ownedbyF.Hoffmann-LaRocheLtd;andbyUnitedStatesPatentNos.5,538,84
