2、高（P＜0.05），细胞的增殖速度显著加快。另外，天然脑活素连续培养的细胞在老龄阶段衰老细胞数显著低于老龄对照细胞（P＜0.01）。结论：天然脑活素可升高MRC-5细胞增殖能力，有效延缓细胞的复制性衰老。【关键词】 天然脑活素；人胚肺成纤维细胞；增殖活度；复制性衰老；延缓 〔Abstract〕 Objective To explore the effects of Natural Cerebrolysin on cell proliferation activity andreplicat
3、ive senescence of human embryonic lung diploid fibroblasts (MRC-5 cells) and its mechanism. Methods To observe the changes of senescent phenotype by light microscope, count the passageages and velocity, detect the potential of cell proliferation by MT
4、T. The changes of cells senescenceof Natural Cerebrolysin-effected MRC-5 cells were studied by X-Gal staining method, and the positivecell rates of senescence cells were detected and analyzed.． Results Natural Cerebrolysin maintainedunaltered cell m
5、orphology and increased life span of MRC-5 by at least 18-21 passages. Comparing withthe control senium cells, the proliferation potential of MRC-5 cells incubated with Natural Cerebrolysinwas increased obviously (P < 0.05), and the passage velocity was
6、enhanced double of that of the controlsenium cells (P < 0.01). In addition, the positive cell rate of senescence cells was significantly lower inlate passage cells in sequenced multigeneration culture in Natural Cerebrolysin than those of control seniumc
7、ells (P < 0.01), and similar to those of young cells. Conclusion As a result, we concluded that Natural Cerebrolysin could notably promote the proliferation activity of MRC-5 cells and delay the cells replicative senescence efficiently. 〔Key Words
