英汉对照分子生物学导论

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1、Chapter4TranscriptioninEukaryotes:MechanismandRegulation4.1EukaryoticRNApolymerases4.2EukaryoticPromoters4.3GeneralTranscriptionFactorsandInitiation4.4SpecificTranscriptionFactorsandTranscriptionalRegulation4.5Structuresofspecifictranscriptionfactors4.6Expe

2、riments4.1真核生物RNA聚合酶4.2真核生物启动子4.3通用转录因子与转录起始4.4特异转录因子与转录调控4.5特异转录因子的结构4.6实验研究TranscriptioninEukaryotes:MechanismandRegulation真核生物转录：机理与调控ProkaryoteEukaryoteTranscriptioninprokaryotesTranscriptionineukaryotesEukaryoticRNApolymeraseIIandtranscriptionfactorsSp

3、ecifictranscriptionfactorGeneraltranscriptionfactorsGeneraltranscriptionfactors4.1EukaryoticRNApolymerases真核生物RNA聚合酶LocationsofRNApolymerasesNucleolus(RNApolymeraseI)Nucleoplasm(RNApolymeraseIIandIII)RNApolymeraseIRNApolymeraseIrRNAprecursorProcessing5.8SrR

4、NA18SrRNA28SrRNARNApolymeraseIIIRNApolymeraseIIIRNAprecursorProcessing5SrRNA4.5StRNAU6snRNARNApolymeraseIIRNApolymeraseIIRNAprecursorProcessingsnRNAsTranslationProteinmRNARNApolymeraseIIHoloenzyme:Coreenzyme(β’,β&α)+SubunitσRNApolII:Coresubunits(1,2&3)+Subu

5、nits4-12RNApolymeraseII4.2EukaryoticPromoters真核生物启动子ProkaryoticpromoterEukaryoticpromoterEukaryoticPromoters真核生物启动子“IwouldliketohaveTATAboxandInitiatorasmypromoter.Whatwouldyoulike?”“Well,IpreferBREandDPE.”TATAboxTATAAA-10boxTATAATSpecializedgeneshaveTATAbo

6、xesTATAboxSpecializedgenes+Housekeepinggenes–Genesofdevelopment–DPE:DownstreamPromoterElementGCGATInitiator/起始子PyPyANPyPyTABRE:TFIIBRecognitionElementCGCCGGGCCA4.3GeneralTranscriptionFactorsandInitiation（通用转录因子与转录起始）ProkaryoticRNApolymerasepre-initiationco

7、mplexEukaryoticRNApolymeraseII:“Ineedhelpfromotherproteins.”4.3.1TFIIDTBP:TATA-BindingProteinShow3DstructureofTBPFile:TBP-TATAboxcomplex1.valTAFIIs:TBP-AssociatedFactorsIITAFIIs:1)BindingtoDPEorInrTAFIIs:2)BindingtoActivator4.3.2OtherTFIIsNowshowingInitiati

8、onofTranscriptionFile:biophoto7LifeInitiationoftranscription(5’)4.3.3GeneralTranscriptionfactorsforRNAPolymeraseIandIIIRNA聚合酶I和III的通用转录因子1.GeneralTFsofRNApolymeraseI2.GeneralTFsofRNApolymeraseIIIGen