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1、上海大学硕士学位论文食品中残留毒物分析方法研究姓名：樊祥申请学位级别：硕士专业：物理化学指导教师：吕敬慈;方晓明20050601上海大学硕士学位论文2.LC/Q—TOFMS法对鸡肉、猪肝中10种p一兴奋剂的测定方法。样品经P一葡萄糖酶过役解离。鸡肉样品经过酸化甲醇提取后，用C18小柱、OasisMCX小柱浓缩、净化。猪肝样品经5%高氯酸提取，调节pH至9.5〜10,乙酸乙酯一异丙醇(6：4,v/v)液液分配，再经小柱净化。ZorbaxXDB—C18色谱柱(2.1xl50m邑谱分离P流刼和为AMeOH.H20(6:4,v/v),BMe
2、OH-H20(1:9,v/v),摊鷹洗脫，16mL/mino样品平均回收率为60〜85%,相对标准偏差为8--14%,检测限为1n£/g。第四章液相色谱.四极杆串联质谱测定高温烘烤、煎炸食品屮内烯酰胺的含量。样品经纯水提取、高速离心，过Maxi.Spin(0.451amPVDF)滤膜，用0asi和目bBdE1mAccucat固相萃取柱净化。ZorbaxXDB一C18(2.1X150mm,焦分离？魏蔚相为MeOH.H20(1:99,v/v)含0・1%HAc溶液，流速0•19mL/mi电喷雾正离子MRM模式检测：丙烯酰胺m/z72斗55
3、,内标m/z75斗58。内标法定量，方法定量检测限为50ng/go第五章总结了本文工作的技术难点及创新点。关键词：高效液相色谱；四极杆.飞行时间质谱；串联四级杆质谱；荧光检测；贝W;B—兴奋剂；丙烯酰胺上海大学硕士学位论文ABSTRACTfoodsafety“Ereadisthestaftofliren.iSahotproblemarousingperna11ryessentia1todevelopsrapidandaccuratemethdsniaamteshecosnemepm•IAeovt•11Qtrcaehfyfro1aet
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