一株中度嗜盐细菌whb45的鉴定及其抗菌与抗肿瘤活性筛选

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1、一株中度嗜盐细菌whb45的鉴定及其抗菌与抗肿瘤活性筛选陈雷王光玉$卜同2张允斌2刘明I张军“林秀坤厂(1.中国科学院海洋研究所实验海洋生物学重点实验室山东青岛266071)(2.哈尔滨工业大学(威海)海洋学院山东威海264209)(3.中国科学院研究牛院北京100039)(4.山东省医药工业设计院山东济南250013)摘要：从盐场中分离鉴定中度嗜盐细菌并对其潜在的抗菌和抗肿瘤活性进行评价。从山东威海的鹿道口盐场分离嗜盐细菌，对菌株whb45进行形态学和生理生化特性研究，测定其16SrRNA序列并通

2、过同源性比对进行系统发育分析，采用抗菌和细胞毒模型进行活性筛选。试验结果表明，菌株whb45为中度嗜盐细菌，whb45与Halobacillustrueperi在形态和生理生化特征方面最接近，16SrRNA序列相似性为99%。whb45的粗提物对多种细菌、真菌和肿瘤细胞的生长都具有较强的抑制作用，可以作为发现生物活性物质的潜在的新来源。关键词：中度嗜盐细菌，鉴定，喜盐芽彳包杆菌属，抗菌活性，抗肿瘤活性IdentificationofaModeratelyHalophilicBacteriumwhb4

3、5andScreeningofItsAntimicrobialandAntitumorActivityCHENLei123WANGGuang-Yu2BUTong13ZHANGYun-Bin2LIUMing1ZHANGJun4LINXiu-Kun1*(1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences,Qingdao,Shandong266071,China)(2.Colle

4、geofMarineScience,HarbinInsliluleofTechnologyatWeihai,Weihai.Shandong264209,China)(3.GraduateSchool、ChineseAcademyofSciences.Beijing100039,China)(4.ShandongProvinceDesignInstituteofPharmaceuticalIndustry,Jinan9Shandong250013,China)Abstract:Theaimofthis

5、studywastoisolateandidentifymoderatelyhalophilicbacteriafromsolarsalternandtoinvestigatetheirpotentialantimicrobialandantitumoractivities.HalophilicbacteriawereisolatedfromLudaokouSolarSalterninWeihaiofShandongProvince.Theidentificationofthestrainwhb45

6、wasdetenninedbytheirmorphologicalcharacteristics,physiologicalandbiochemicaltestsandphylogeneticanalysisbasedon16SrRNAsequencecomparison.Theantimicrobialactivityandcytotoxicactivitywerescreened・Ourresultsshowedthatthestrainwhb45wasbelongedtomoderatelyh

7、alophilicbacterium.Phylogeneticanalysisshowedthestrainwhb45hada99%homologywithHalobacillustrueperi.Thecrudeextractfiomwhb45showedstrongerantimicrobialactivitieswhichcouldinhibitthegrowthofseveralbacteriaandfungi.MTTanalysisrevealedthatthestrainpossesse

8、dpotentanticanccractivitywiththestrongerinhibitoryeffectonseveralhumancancercells・Ourresultssuggestedthatthemoderatelyhalophilicbacteriumwhb45maybedevelopedasapotentialnewsourceforthediscoveryofnovelbioactivcsubstances.Keywords:Moderate