一株中度嗜盐蜡样芽孢杆菌的分离鉴定及其对苯胺的降解特性

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1、一株中度嗜盐蜡样芽砲杆菌的分离鉴定及其对苯胺的降解特性一株中度嗜盐蜡样芽抱杆菌的分离鉴定及其对苯胺的降解特性摘要：从江苏省连云港市某化工染料厂排污管道的污水中驯化、分离得到1株能以苯胺为惟一碳源的降解菌株HYHB-3,经形态学特征及生理生化特性观察，结合16SrRNA基因序列分析，初步鉴定为蜡样芽砲杆菌(Bacilluscereus),并研究其对苯胺的降解特性和影响因素。结果表明，菌株HYHB-3为该菌株属屮度嗜盐，最佳生长温度25°C,超过35°C停止生长；苯胺浓度100mg/L是HYHB-3菌株降解最适浓度，降解率达77.9%,250mg/L为饱和浓度；随时间延长降解效率有增加趋

2、势；菌株HYHB-3降解苯胺的最适温度是25°C,降解率达69.9%,II对低温条件具有一定的适应性，15〜20°C条件下均有一定的降解；降解苯胺最适pH为8,降解率达到最大值，达58.4%；在NaCl含量1%〜9%的范围内均有一定的降解，3%、5%时降解率均超过50%以上，9%时仍然有11.7%的降解率。苯胺浓度250mg/L以上则可以抑制该菌的生长。关键词：蜡样芽抱杆菌(Bacilluscereus)；分离鉴定；苯胺；降解中图分类号:S852.61+6文献标识码:A文章编号:0439-8114(2014)02-0308-04IsolationandIdentificationof

3、aModeratelyHalophilicBacilluscereusandItsDegradationCharacteristiconAnilineWANGHong-binl,2,SONGXiu-meil,ZHENGJin-longl,YANBin-lun2(1.MarineCollege,HuaiHaiInstituteofTechnology,Lianyungang222005,Jiangsu,China；2.JiangsuKeyLaboratoryofMarineBiotechnology,Lianyungang222005,Jiangsu,China)Abstract：Ah

4、ighlyefficientdegradationbacteriumHYHB-3withanilineasitssolecarbonsourcewasisolatedfromthesewagepipeofachemicaldyefactoryinLianyungang,JiangsuProvince・Accordingtothemorphological,physiologicalandbiochemicalcharacteristics,combinedwith16SrRNAsequenceanalysis,itwasidentifiedasBacilluscereus・Itsde

5、gradationcharacteristicsonanilineandfactorsinfluencingthedegradationwerestudied・TheresultsshowedthatthestrainIIYIIB-3wasmoderatelyhalophilicwithoptimumgrowthtemperatureof25°C.Itmightstopgrowingover35°C.Theanilinemassconcentrationof100mg/LwastheoptimaldegradationconcentrationofstrainHYHB-3,witht

6、hehighestdegradationrate77.9%.250mg/Lwasthesaturationconcentration.Theoptimumdegradationtimewas96〜120h・Thedegradationefficiencyincreasedwithtime・TheoptimumtemperatureofanilinedegradationofStrainIIYIIB-3was25°Cwithdegradationrate69.9%.Ithadcertainadaptabilitytothelowtemperatureconditionwith15~20

7、°C.TheoptimumplIofanilinedegradationwas8withmaximumanilinedegradationrate58.4%.TherewasacertaindegradationwhentheNaClcontentof1%~9%.Thedegradationratewereover50%whenNaClwas3%and5%.Thedegradationratewas11.7%whenNaClwas9%.Theaniline