镍柱纯化蛋白说明书

《镍柱纯化蛋白说明书》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、InstructionManualProBondTMPurificationSystemForpurificationofpolyhistidine-containingrecombinantproteinsCatalognos.K850-01,K851-01,K852-01,K853-01,K854-01,R801-01,R801-15VersionK2September200425-0006iiTableofContentsKitContentsandStorage............................................

2、......................................................................................ivAccessoryProducts...........................................................................................................................................viIntroduction.......................

3、..........................................................................................................1Overview..............................................................................................................................................................1Methods

4、.......................................................................................................................................2PreparingCellLysates.............................................................................................................................

5、...........2PurificationProcedure—NativeConditions....................................................................................................7PurificationProcedure—DenaturingConditions........................................................................................

6、.11PurificationProcedure—HybridConditions.................................................................................................13Troubleshooting..............................................................................................................................

7、...................15Appendix....................................................................................................................................17AdditionalProtocols...................................................................................................

8、.................................