镍柱纯化-HisTrap-HP

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1、71502768_AH_v1.fmPage1Wednesday,April15,20091:43PMGEHealthcareInstructions71-5027-68AHHisTrapaffinitycolumnsHisTrapHP,1mland5mlHisTrap™HPisareadytousecolumn,prepackedwithprechargedNiSepharose™HighPerformance.Thisprepackedcolumnisidealforpreparativepurifica

2、tionofHistidine-taggedrecombinantproteinsbyimmobilizedmetalionaffinitychromatography(IMAC).Thespecialdesignofthecolumn,togetherwiththehigh-performancematrixoftheNiSepharosemedium,providesfast,simple,andeasyseparationsinaconvenientformat.NiSepharoseHighPerf

3、ormancehaslownickel(Ni2+)ionleakageandiscompatiblewithawiderangeofadditivesusedinproteinpurification.HPcolumnscanbeoperatedwithasyringe,peristalticpump,orliquidchromatographysystemsuchasÄKTA™designchromatographysystems.CAUTION!Containsnickel.Mayproduceanal

4、lergicreaction..71502768_AH_v1.fmPage2Wednesday,April15,20091:43PMCodeNo.ProductNo.supplied17-5247-01HisTrapHP5×1ml17-5247-05HisTrapHP100×1ml117-5248-01HisTrapHP1×5ml17-5248-02HisTrapHP5×5ml17-5248-05HisTrapHP100×5ml11Packsizeavailablebyspecialorder.Connec

5、torkitConnectorsUsageNo.suppliedsupplied1/16”male/Connectionofsyringetotopof1luerfemaleHiTrapcolumnTubingconnectorConnectionoftubing(e.g.Peristaltic1flangeless/PumpP1)tobottomofHiTrapM6femalecolumn1TubingconnectorConnectionoftubing(e.g.Peristaltic1flangele

6、ss/M6malePumpP1)totopofHiTrapcolumn2Union1/16”ConnectiontooriginalFPLC™1female/M6maleSystemthroughbottomofHiTrapcolumnUnionM6female/ConnectiontooriginalFPLCSystem11/16”malethroughtopofHiTrapcolumnStopplugfemale,SealingbottomofHiTrapcolumn2,5or71/16”1Union1

7、/16”female/M6maleisalsoneeded.2UnionM6female/1/16”maleisalsoneeded.Tableofcontents1.Description..............................................................................32.Generalconsiderations.....................................................63.Ope

8、ration.................................................................................74.Optimization........................................................................115.Strippingandrecharging........