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1、XX大学毕业论文温肾健脾方对大鼠慢性创面愈合的影响2014年6月25日温肾健脾方对大鼠慢性创面愈合的影响作者：曹永清，何春梅，陆金根【关键词】创面愈合［摘要］目的：探讨温肾健脾中药对慢性创面愈合的影响及可能作用机制。方法：采用大鼠背部开放性创而模型，用肌注氢化可的松的方法造成慢性难愈性创面，以辛葡康为阳性对照约，应用免疫组化、流式细胞术等方法，观察创面愈合吋间、新生上皮宽度、肉芽组织形态学变化、肉芽组织细胞周期、表皮生长因子(epidermalgrowthfactor,EGF)转化生长因子卩1(transforminggr
2、owthfactor卩1,TGF卩1)及纤维连接蛋fl(fibronectin,FN)^指标。结果：温肾健脾方治疗组与辛葡康对照组平均创面愈合时间分别为(17.0±1.9)和(18.8±1.9)d,较模型组及空白组明显缩短(P0.05)；治疗14d后，治疗组及对照组新生上皮宽度分别为(3・73±0・19)和(3.21±0.15)mm,较模型组及空白组明显增宽(P0.05)；治疗组新生毛细血管丰富，成纤维细胞数量多，而对照组、模型组及空白组新生毛细血管及成纤维细胞数量均较治疗组明显减少(P0.05)；治疗组S期细胞比例较对照
3、组、模型组及空白组明显捉高(P0.05)；治疗组及对照组与模型组及空白组比较，EGF、TGFpkFN蛋白表达均增强(P0.05)。结论：温肾健脾方有促进人鼠慢性难愈创面修复的作用，其机制与调节细胞周期，上调EGF、TGFpl细胞因子蛋白表达水平，促进间质FN的表达有关。［关键词］中药疗法；创而愈合；肉芽组织；组织学比较；细胞因子EffectsofWenshenJianpiRecipeonchronicwoundhealinginratsABSTRACTObjective:TostudytheeffectsofWenshen
4、JianpiRecipe(WSJPR,atraditionalChinesemedicineforwarmingkidneyandinvigoratingspleen)onchronicwoundhealingandthemechanism.Methods:NinetysixSDratswererandomlydividedinto4groups,with24ratsineachgroup,andbackwoundwasmadeintherats.Forratsin3ofthe4groups,hydrocortisonei
5、njectionwasadministeredtoinducechronicwound.Ratsin2ofthe3groupsweretreatedwithWSJPRandXinpukangGranules(XPKG)respectively,andtheratsintheothergroupwereuntreated.Theratsinthefourthgroupweretakenascontrol.Thewoundhealingtimeandthewidthofnewepidermiswereobserved,andt
6、hehistomorphologicalchangesandcellcycleofthegranulationtissue,andtheproteinexpressionsofepidermalgrowthfactor(EGF)，transforminggrowthfactor卩1(TGF卩1)andfibronectin(FN)inthegranulationtissueweretestedwithimmunohistochemicaltechniqueandflowcytometry.Results:Thewoundh
7、ealingtimeoftheWSJPRtreatedandXPKGtreatedgroupswas(17・0±L9)and(18.8±1.9)drespectively,muchshorterthanthatoftheuntreatedandcontrolgroups(P0.05).Onthe14thexperimentday,thewidthofnewepidermisoftheWSJPRtreatedandXPKGtreatedgroupswas(3・73±0・19)and(3.2H0.15)mmrespective
8、ly,muchwiderthanthatoftheuntreatedandcontrolgroups(P0.05).Thenumbersofangiogenesis,fibroblastsandcellsintheSphaseinWSJPRtreatedandXPKGtreatedgroupswerem
