asodn转染骨肉瘤细胞对survivin及vegf表达的影响

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1、重庆医科大学硕士研究生学位论文THEEFFECToNTHEEXPRESSIONOFSURVIVINANDVEGFINoSTEOSARCoMACELLSAFTERTHETRANSFECTIONoFASODNABSTRACTObjective：TostudytheexpressionanditsrelationshipofSurvivingeneandVEGFgeneintheosteosarcomacell(9607cellline)，aswellasthecellproliferationafterth

2、einterferenceofASODN．SoastoapproachtheinteractionofSurvivingeneandVEGFgeneinosteosarcomacell，whichwillprovidesomecluesfortheoccurrence，development，metastasisoftheosteosarcoma．Method：Theosteosarcomacells(9607cellline)wereculturedanddividedintoexperimental

3、groupandcontrolgroup．SurvivinandVEGFphosphorothioateASODNwastransfectedinto9607cellsbylipofectamine2000respectivelyinexperimentalgroup，meanwhile9607cellsincontrolgroupwastreatedbysimplelipofectamine．Theinhibitionratioof9607cellsindifferentgroupsweredeter

4、minedbyMTT，theexpressionofSurvivinmRNAandVEGFmRNAweredeterminedbyRT—PCR，theexpressionofSurvivinproteinandVEGFproteinweredeterminedbyimmunocytochemistry．Results：(1)AfterthetransfectionofSurvivin—ASODN，theinhibition5重庆医科大学硕士研究生学位论文ratioof9607cellsat24hours

5、，48hoursand72hourswas(31．400±O．691)％，(52．515±O．870)％，(78．473±1．025)％respectively．AfterthetransfectionofVEGF—ASODN，theinhibitionratioof9607cellsat24hours，48hoursand72hoursWas(28．511±0．825)％，(49．292±0．900)％，(71．349-1-1．223)％respectively．Theinhibitionratioo

6、f9607cellsinSurvivin··ASODNgroupandVEGF--ASODNgroupwerehigherthanthatincontrolgroupindifferenttimepoints，P<0．05．(2)TheexpressionofSurvivinmRNAin9607cellsofSurvivin—ASODNgroupandVEGF—ASODNgroupwas(1．5198士0．084)and(1．6261士0．091)respectively，whichwerelowert

7、hanthatofcontrolgroup(1．9610士0．094)，P<0．05．TheexpressionofVEGFmRNAin9607cellsofSurvivin—ASODNgroupandVEGF—ASODNgroupwas(1．748910．065)and(1．5429土0．064)respectively，whichwaslowerthanthatofcontrolgroup(2．0453士0．082)，P<0．05．TheexpressionofSurvivinmRNAandVEGF

8、mRNAwerebothdecreasedinSurvivin—ASODNgroupandVEGF—ASODNgroup．TheinhibitionratiooftheexpressionofSurvivinandVEGFmRNAwaspositivelycorrelatedinSurvivin—ASODNgroup(r=O．602)andVEGF-ASODNgroup(r=0．702)．(3)Theappearanceofcellsinc