可卡因胶体金诊断试剂用原料研发

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1、ABSTRACTCocaineismainlyusedforsurfaceanesthesia,withastrongcentralnervoussystemstimulant,takingasusual,theaddictive,atpresent,cocaineabuseisgettingworse.Thecompanyhasdevelopedcocainediagnostictest,butthecocaineantigenandantibodydependentonimports,thepriceofimportreag

2、entisexpensive,andtherefore,developourowncocaineantigenandantibody,reduceproductcosts,achievefullproduction,italsohasimportanteconomicsignificanceandsocialvalueoThispapermainlyresearchcocaineN-site・AddcarboxylgrouponN-sitebyorganicreaction,conjugateacarrierproteintoo

3、btaincocaincconjugateantigen.Thenimmunizedmice,obtainanti-cocainemonoclonalantibody.Confirmtheproductionprocessofthisnewcocaineantigenandantibody.Evaluatetheperformanceofthecocainediagnostictestwithnewantigenandantibody.TheexperimentresultsshowedthatoncocaineNsite,ad

4、daceticacid,usingDCC+NHSmethodinph7.4buflcrunder2〜8°Ctemperatureconjugate5mg/mlbovinesemmalbumin,cansuccessfullyobtainedthecocaineconjugateantigen.Thisantigenhaswellspecificitywiththeimportcocaineantibody.Andthisantigenhasgoodimmunogenicity.Weuseitimmunizedthemice,af

5、tercellfusion,screening,cloning,getwellspecificitycocainemonoclonalantibody.AndnospecificwithBUP,THC,AMP,MET,BZO,MOP,etc.Thenewcocainemonoclonalantibodyproductionprocess:cellculturedensityis8x104/permouse,adjuvantforinjectionisamixtureofparaffinandlanolin,injectcella

6、mountis1.0x106/permouse,andantibodypurificationmethodisn-Caprylicacid-Ammoniumsulfateprecipitation.Thespecificity,sensitivity,testrange,accuracyandclinicalexperimentofthenewcocainerapiddiagnostictestmeettheproductrequirements,andwithnocrossreactivityofvarioussimilar

7、drugs,theclinicalsampletestresultsis99.4%betterthanthecurrenttestimportedreagentlabeled98.8%.Thecostofthenewcocaineantigenandantibodyis20%ofimportsreagent,replacingcangreatlyreducingproductcosts・Keywords:cocaineconjugateantigen,cocainemonoclonalantibody,cocainerapidd

8、iagnostictest第一章绪论11.1可卡因背景知识11.2可卡因适用范围及药理1121适用范围11.2.2药理学11.2.3用法和用量21.2.4药理动力子及体内代谢21.2.5毒性及不良反应21.3可卡因滥用及检测31.3.1可卡因滥用31.3.2可卡