棉花T-DNA插入突变体库构建

《棉花T-DNA插入突变体库构建》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、硕士学位论文MASTER’SDEGREEDISSERTATION棉花T-DNA插入突变体库构建CONSTRUCTIONOFCOTTONT-DNAINSERTIONPOPULATION研究生：刘卫龙CANDIDATE:WEILONGLIU导师：金双侠副教授SUPERVISOR:PROFSHUANGXIAJIN学位类型：农业推广硕士DEGREETYPE:MASTERDEGREEINAGRICULTURALEXTENSION领域：作物FIELD:CROP中国·武汉WUHAN，CHINA二O一四年六月JUNE，2014万方数据学校名称：华中农业大学单位代码：10504分类号：密级：华中农业大学硕

2、士学位论文棉花T-DNA插入突变体库构建CONSTRUCTIONOFCOTTONT-DNAINSERTIONPOPULATION研究生：刘卫龙学号：2012301120008指导老师：金双侠副教授学位类型：农业推广硕士领域：作物华中农业大学植物科学技术学院中国.武汉CollegeofPlantSciencesandTechnologyHuazhongAgriculturalUniversityWuhan,China万方数据万方数据棉花T-DNA插入突变体库构建目录摘要-------------------------------------------------------------

3、------------------------------------iAbsract------------------------------------------------------------------------------------------------ii缩略表-----------------------------------------------------------------------------------------------iii1综述---------------------------------------------------

4、---------------------------------------------11.1植物功能基因组研究······································································11.1.1全基因组测序和基因注释···························································11.1.2植物功能基因组研究手段····························································21.2Genetrap技术在植

5、物功能基因组研究中的应用······································51.2.1几种基因陷阱的原理·································································51.2.2启动子陷阱技术·······································································61.3几种T-DNA插入侧翼序列的分离方法················································71.3.1传统的TAIL

6、-PCR·····································································81.3.2FPNI-PCR················································································81.3.3FPNI-PCR与TAIL-PCR对比························································91.3.4本课题研究意义以及技术路线······························

7、······················122实验材料和方法--------------------------------------------------------------------------------142.1实验材料·····················································································142.1.1品种···