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1、IntroductionsandApplicationsofCellFunctionAssayJinHAOApplicationSpecialist,BDBiosciencesJin.Hao@bd.com©2016BD.BD,theBDLogoandallothertrademarksarepropertyofBecton,DickinsonandCompany.1MaxwellW.BectonFairleghS.DickinsonBD,foundedin1897,isaglobalmedicaltechnologycompanyt
2、hatisadvancingtheworldofhealthbyimprovingmedicaldiscovery,diagnosticsandthedeliveryofcare.BDhasmorethan45,000associatesacross50countrieswhoworkinclosecollaborationwithcustomersandpartnerstohelpenhanceoutcomes,lowerhealthcaredeliverycosts,increaseefficiencies,improvehea
3、lthcaresafetyandexpandaccesstohealth.2©2016BD.BD,theBDLogoandallothertrademarksarepropertyofBecton,DickinsonandCompany.标本分析前处理系统PAS给药流程解决方案生物科学MPSBDB诊断系统呼吸解决方案DSRS糖尿病护理药物制备及分发技术DCDPT制药系统PS3©2016BD.BD,theBDLogoandallothertrademarksarepropertyofBecton,DickinsonandCompany
4、.BDFACSCelestaResearchplatform10C12C14Csolution4©2016BD.BD,theBDLogoandallothertrademarksarepropertyofBecton,DickinsonandCompany.CitationsofFlowCytometers5©2016BD.BD,theBDLogoandallothertrademarksarepropertyofBecton,DickinsonandCompany.BDPharmingenTotalSolutionCellfunc
5、tionassayT/BcellresearchCBA-CytometricBeadArrayBDPharmingenStemCellResearchBDPhosflowIMagCellSeparationSystemWB/IHC/IP/IF/BioimagingELISA/ELISOPT6©2016BD.BD,theBDLogoandallothertrademarksarepropertyofBecton,DickinsonandCompany.BDPMGProduceLine7©2016BD.BD,theBDLogoandal
6、lothertrademarksarepropertyofBecton,DickinsonandCompany.CellFunctionAssayLandscapePhosphatidylSerineExposure556570FITCAnnexinVApoptosisKitLiveCellsMitochondrialMembranePotential551302MitoScreenJC-1ApoptosisCaspaseactivity550914PEActiveCaspase-3ApoptosisKitFixedCellsDNA
7、Fragmentation556405APO-BRDUKitMembraneIntegrity5599257-AADLiveCellsCellularProcessViabilityEsteraseActivity562247FixableViabilityStain450FixedCellsDNAContent561908Hoechst33342SolutionLiveCellsProliferationDyeRetention565082CFSEProliferationKi-67Status562899BV421MouseAn
8、ti-Ki-67andCellCycleDNAContent340242CycletestPlusDNAReagentKitFixedCellsBrdUIncorporation557892APCBrdUFlowKitCyclinEx
