6、增检测模板完整情况。4.通过已发表的贻贝的M7lysin的基因序列,设计兼并性引物扩增M7lysin基因。四、参考文献[1].Vacquier V D. Evolution of gamete recognition protein [J]. Science, 1998, 281(5385): 1995-1998.[2]. Mozingo N M, Vacquier V D, Chandler D E. Structural features of the abalone egg extracellar matrix and it
7、s role in gamete in-teraction during fertilization [J]. Mol Rep And Devel, 1995,41: 493-502.[3]. Swanson W J, Vacquier V D. The abalone egg receptor for sperm lysine is a giant multivalent molecule [J]. Proc Natl Acad SciUSA, 1997, 94: 6724-6729.[4].Swanson W J, Vacqu
8、ier V D. Extraordinary divergence and positive Darwinain selection in a insagenic protein coating the acrosomal process of a