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1、1、EFFECTSOFTHEGROWTHRETARDANTPACLOBUTRAZOLONLARGE-SCALEMICROPROPAGATIONOFDAYLILY(HEMEROCALLISSPP.)JIANXINCHEN*,DAWNE.HALL,ANDVINCENZODELUCA大规模生长延缓剂多效唑影响黄花菜快繁（萱草SPP。）建新陈*，黎明E.霍尔和Vincenzo德卢卡DepartmentofBiology,BrockUniversity,500GlenridgeAvenue,St.Catharines,OntarioL

2、2S3A1,Canada(Received6April2004;accepted5September2004;editorR.D.Shillito)格伦里奇大道500号，圣凯瑟琳，安大略省ł2S3A1，加拿大布鲁克大学，生物系，（2004年4月接受2004年9月编辑RD希利托）SummaryMeristematicclusterswereinducedfromdaylilyscapeexplants(pedicel–scapejunction)inthepresenceofthegrowthretardantPaclobut

3、razolonsemisolidagarmedium.Liquidshakeculturewasusedtoproliferatemeristematicclusters.Highlyefficientregenerationofadventitiousshootsoccurredonclustersaftersubcultureona0.8%agarstrengthsemisolidmediumwiththeadditionofactivatedcharcoal.Paclobutrazolandsucroselevelsi

4、nthemediawerefoundtosignificantlyaffectstarchaccumulation,growthvalue,anddryweightpercentageofliquid-culturedmeristematicclusters.Theuseofliquidshakeculturesformassproliferationofmeristematicclustersfollowedbyregenerationofadventitiousshootsonsemisolidagarcultureco

5、uldbeanefficientsystemforlarge-scalemicropropagationofdaylily.总结根尖诱导集群从黄花菜景观植（花梗景观路口）半固体琼脂培养基上生长延缓剂多效唑的存在。液体振荡培养物用于增殖分生集群。高效再生不定芽发生在0.8％的琼脂强度与另外活性炭半固体培养基继代集群。多效唑和蔗糖水平在媒体上被发现显着影响淀粉的积累，成长值，液体分生组织培养集群干重量百分比。液体振荡培养大量增殖半固体琼脂培养不定芽再生的分生组织集群使用可能是一个有效的黄花菜进行大规模快繁体系。Keywords:

6、daylily;Hemerocallis;growthretardant;liquidculture;micropropagation.关键词：黄花菜，黄花菜生长延缓剂，液体培养快繁技术。2、SpentmediumanalysisforliquidculturemicropropagationofHemerocallisonMurashigeandSkoogmedium分析了介质为液体培养快速繁殖的萱草的Murashige和Skoog介质JeffreyW.Adelberg&MariaP.Delgado&JefferyT.To

7、mkinsReceived:14July2008/Accepted:20August2009/Editor:T.J.Jones#TheSocietyforInVitroBiology2009杰弗里·Adelberg玛丽亚·P.德尔加多＆杰弗里T.汤姆金斯收稿日期：2008年7月14日/接受日期：2009年8月20日/编辑：TJ琼斯2009年在体外生物学学会＃AbstractResidualnutrientsfromMurashigeandSkoogmediumwereanalyzedfollowinga5-wkmultifa

8、ctorexperiment.Plantdensity,sugarconcentration,andplantgrowthregulators(benzyladenineandancymidol)wereexaminedusingfourgenotypesofdaylily(Hemeroc