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1、稀有放线菌的分离与培养报告人：张晓敏报告内容分离培养基的选择小单孢菌形态与生理生化特征拟诺卡氏菌形态与生理生化特征小单孢菌、拟诺卡氏菌与链霉菌区别分离培养基的选择淀粉-酪蛋白硝酸盐琼脂培养基溶胶几丁质琼脂培养基甘露醇-利福平琼脂培养基淀粉-酪蛋白硝酸盐琼脂培养基starch-caseinnitrateagar：starch10g，sodiumcaseinate0.3g，KNO32g，agar15g，PH7.0-7.4.37μgnovobiocin，223μgmycostatin30℃培养21天Micromonosporachaiyaphumensissp.novMicrom
2、onosporaeburneasp.nov基础培养基：K2HPO40.7g，KH2PO40.3g，MgSO4·7H2O0.5g，FeSO4·7H2O0.01g，ZnSO40.001g，琼脂10克，蒸馏水1.0L.PH7.0121℃蒸汽灭菌20min,每100mL加20mL胶体几丁质悬浮液和5mg放线菌酮溶胶几丁质琼脂培养基甘露醇-利福平琼脂培养基mannitol-rifampicinagar：甘露醇10g，酵母提取物7g，酪蛋白水解物2g，蛋白胨1g，NaCl1g，CaCO30.2g，制霉菌素100mg,利福平5mg27℃培养6周Nocardiaacidivoran返回Mi
3、cromonosporagyrB基因序列分析和DNA–DNA杂交15species:Micromonosporaaurantiaca,Micromonosporacarbonacea,Micromonosporachalcea,M.chersina,Micromonosporacoerulea,Micromonosporaechinospora,Micromonosporagallica,Micromonosporahalophytica,Micromonosporainositola,M.matsumotoense,Micromonosporanigra,M.olivas
4、terospora,Micromonosporapallida,MicromonosporapurpureochromogenesandM.rosaria.小单孢菌形态与生理生化特征Aerobic,Gram-positive,mesophilic,non-motileactinomycetewell-developedandbranchedsubstratehyphae.Aerialmyceliumwasabsent.Sporechainswereabsent.Mycolicacidswereabsent.Acyltypeofcellwallmuramicacidisgly
5、colyl.Fatty-acidtype:3b.Thepredominantmenaquinone:MK-9（H4）、MK-9（H4H6H8）Characteristicwhole-cellsugarsarexyloseandarabinose.Phospholipids:P-II型.Cell-wallpeptidoglycan:CW-II(glutamicacid,alanineandmeso-diaminopimelicacid).DNAG+C(mol%):71%-73%.>well-developedandbranchedsubstratehyphae.>Aerial
6、myceliumwasabsent.>Atmaturity,sporesweresphericaltooval,appearedtoberoughandwerenon-motile(Fig.1).>colourofthevegetativemyceliumonISP2mediumisorange,turningtogreyishblackorblackuponsporulation.>growthatpH4-8and25-30℃,nogrowthabove45℃>Brown,diffusiblepigment>positivefortheutilizationofL-ara
7、binose,D-fructose,D-galactose,D-glucose,lactose,melibiose,salicinandD-xylose.>negativefornitratereductionandtheutilizationofD-mannitolandrhamnose.Micromonosporachaiyaphumensis>Single,non-motile,sphericalspores(0.6–0.9mm)wereformedonsubstratehyphae.>Aerialmycel
