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《Development and Evaluation of a Novel Solution, Somahfor the Procurement and Preservation of Beating and,一种新型溶液SOMAH的开发和评价 关于打浆的采购和保存 非搏动供体心脏移植》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、TransplantationDevelopmentandEvaluationofaNovelSolution,Somah,fortheProcurementandPreservationofBeatingandNonbeatingDonorHeartsforTransplantationHemantS.Thatte,MSc,PhD;LakiRousou,MS,MD;BaderE.Hussaini,MD,MPH;Xiu-GuiLu,MD;PatrickR.Treanor,CCP,LCP;ShukriF.Khuri,MD†Background—Injurytomyocytes,
2、endocardium,andthecoronaryendotheliumduringharvestingandstoragecancompromiseoutcomesafterhearttransplantation.Safeguardingofstructureandfunctionofcardiomyocytesandendotheliumindonorheartsmayleadtoimprovedpatientsurvivalaftertransplantation.Informationgainedfromporcineheartsstoredinstandardt
3、ransplantsolutionwasusedtodesignasuperiorpreservationsolutionthatwouldoptimallyprotectandmaintainorgansfrombeatingheartand/ornonbeatingheartdonorsduringlong-termstorage.MethodsandResults—Multiphotonmicroscopywasusedtoimagedeepwithincardiacbiopsiesandcoronaryarterytissueharvestedfromporcineh
4、eartsobtainedfrombeatingheartandnonbeatingheartdonorsforanalysisofmyocyteandendothelialcellstructureandfunction.Cellstructuralintegrityandviability,calciummobilization,andnitricoxidegenerationweredeterminedwithfluorescenceviabilitymarkers,immunofluorescence,andWesternblots.Duringhypothermic
5、storageinstandardpreservationsolution,Celsior,myocyte,andendothelialviabilitywasmarkedlyattenuatedinheartsobtainedfrombeatingheartdonors.Incontrast,heartsfrombeatingandnonbeatingheartdonorsstoredinthenewlyformulatedSomahsolutiondemonstratedanincreaseinhigh-energyphosphatelevels,protectionof
6、cardiacmyocyteviability,mitochondrialmembranepolarization,andstructuralproteins.Similarly,coronaryarteryendothelialorganizationandfunction,calciummobilization,andnitricoxidegenerationwerewellmaintainedduringtemporalstorageinSomah.Conclusions—TheCelsiorpreservationsolutioninclinicalusetodayh
7、asledtoaprofounddeclineincardiomyocyteandendothelialcellviability,whereasthenewlydesignedSomahsolutionhassafeguardedmyocyteandendothelialintegrityDownloadedfromhttp://ahajournals.orgbyonAugust11,2018andfunctionduringorganstorage.UseofSomahasastoragemediu
