正文描述:《2017-NC-Land-locked mammalian Golgi reveals cargo transport between stable cisternae》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、ARTICLEDOI:10.1038/s41467-017-00570-zOPENLand-lockedmammalianGolgirevealscargotransportbetweenstablecisternaeMyunHwaDunlop1,AndreasM.Ernst1,LenaK.Schroeder1,DerekK.Toomre1,GrégoryLavieu1&JamesE.Rothman1TheGolgiiscomposedofastackofcis,medial,transcisternaethatarebio
2、chemicallydistinct.Thestablecompartmentsmodelpostulatesthatpermanentcisternaecommunicatethroughbi-directionalvesicles,whilethecisternalmaturationmodelpostulatesthattransientcisternaebiochemicallymaturetoensureanterogradetransport.Testingeithermodelhasbeenconstraine
3、dbythediffractionlimitoflightmicroscopy,asthecisternaeareonly10–20nmthickandcloselystackedinmammaliancells.WepreviouslydescribedtheunstackingofGolgibytheectopicadhesionofGolgicisternaetomitochondria.Here,weshowthatcargoprocessingandtransportcontinue—evenwhenindivid
4、ualGolgicisternaeareseparatedand“land-locked”betweenmitochondria.Withtheincreasedspatialseparationofcisternae,weshowusingthree-dimensionalliveimagingthatcis-Golgiandtrans-Golgiremainstableintheircompositionandsize.Hence,weprovidenewevidenceinsupportofthestablecompa
5、rtmentsmodelinmammaliancells.1DepartmentofCellBiology,YaleSchoolofMedicine,NewHaven,CT06511,USA.CorrespondenceandrequestsformaterialsshouldbeaddressedtoJ.E.R.(email:james.rothman@yale.edu)NATURECOMMUNICATIONS
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7、DOI:10.1038/s41467-017-00570-z
8、www.nature.com/natu
9、recommunications1ARTICLENATURECOMMUNICATIONS
10、DOI:10.1038/s41467-017-00570-zheGolgiiscomposedofastackofflattenedcisternaethatprogression/maturationmodel1,3.Inthismodel,theGolgicis-servesasanobligateintermediateformembranetraffickingternaitselfisthecargocarrierthatgrad
11、uallyprogresses/maturesTofproteinsinthesecretorypathway1.ProteinsandlipidsfromcistomedialtotransbytheuptakeofretrogradeCOPIenterfromtheendoplasmicreticulum(ER)intothecis-Golgi,vesiclesthatdeliverresidentenzymesfromoldertoyoungerwheretheyundergoextensivemodificationa
12、stheytraversethecisternae.Golgi,includingglycosylation,sulfation,phosphorylation,lipi-CisternalmaturationwasdirectlyvisualizedinS.cerevisiae,dati
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