资源描述：
《2014 Genome biology. Genome-wide profiling of mouse secondary structures reveals key features of the mammalian transcriptome》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、Incarnatoetal.GenomeBiology2014,15:491http://genomebiology.com/2014/15/10/491RESEARCHOpenAccessGenome-wideprofilingofmouseRNAsecondarystructuresrevealskeyfeaturesofthemammaliantranscriptome1,2111,3*DannyIncarnato,FrancescoNeri,FrancescaAnselmiandSalvatoreOlivieroAbstractB
2、ackground:TheunderstandingofRNAstructureisakeyfeaturetowardthecomprehensionofRNAfunctionsandmechanismsofaction.Inparticular,non-codingRNAsarethoughttoexerttheirfunctionsbyspecificsecondarystructures,butanefficientannotationonalargescaleofthesestructuresisstillmissing.Resu
3、lts:Byusinganovelhigh-throughputmethod,namedchemicalinferenceofRNAstructures,CIRS-seq,thatusesdimethylsulfate,andN-cyclohexyl-N'-(2-morpholinoethyl)carbodiimidemetho-p-toluenesulfonatetomodifyRNAresiduesinsingle-strandedconformationwithinnativedeproteinizedRNAsecondarystr
4、uctures,weinvestigatethestructuralfeaturesofmouseembryonicstemcelltranscripts.Ouranalysisrevealsanunexpectedhigherstructuringofthe5′and3′untranslatedregionscomparedtothecodingregions,areducedstructuringattheKozaksequenceandstopcodon,andathree-nucleotideperiodicityacrossth
5、ecodingregionofmessengerRNAs.WealsoobservethatncRNAsexhibitahigherdegreeofstructuringwithrespecttoproteincodingtranscripts.Moreover,wefindthattheLin28abindingproteinbindsselectivelytoRNAmotifswithastrongpreferencetowardasinglestrandedconformation.Conclusions:Thisworkdefin
6、esforthefirsttimethecompleteRNAstructuromeofmouseembryonicstemcells,revealinganextremelydistinctRNAstructurallandscape.TheseresultsdemonstratethatCIRS-seqconstitutesanimportanttoolfortheidentificationofnativedeproteinizedRNAstructures.Backgroundnuclearribonucleicparticle(
7、snRNP)complexes[9,10],Thedevelopmentofhigh-throughputmethodsforthemostncRNAsarethoughttoexerttheirfunctionsbyanalysisoftheepigenomeandtranscriptomehaveledtofoldingintolocallystablesecondarystructuresthatmaythediscoveryofthousandsofpreviouslyunannotatedprovideanchoringsite
8、sforinteractingproteins.Forex-transcripts[1,2],manyofwhichlacktheabilitytoen-ample,ithasbeenshow
