不同类型缺氧Different Types of Hypoxia(英文版)

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1、LabReportofMedicalFunctionalExperimentationDate：2014.5.27TitleDifferentTypesofHypoxiaScoreExperimentersGradeandspecializedfieldPreventiveMedicine，Group101.PurposeA.TounderstandtheclassificationprincipleofhypoxiabyduplicationofanimalmodelofdifferenttypesofhypoxiaB.Tounderstandthemanifesta

2、tioncharacterofdifferenttypesofhypoxiathroughtheobservationofthevariationofrespiratoryrhythmandskinandmucosacolorofanimalwithhypoxia.2.AnimalandMaterials(1)Animal:Adultmice,weighingabout20gram,maleorfemale(2)Apparatus:GinsbergScientificReagentBottles-Flint-WideMouth-GroundGlassStopper,ca

3、rbonmonoxidegeneratingdevice,alcohollamp)、syringe,needles,scissors,tissueforceps.(3)Drugsandreagents:Sodalime,formicacidsolution,concentratedsulfuricacidsolution,5%sodiumnitritesolution,1%methylenebluesolution.3.MethodsandProcedures（1）Hypotonichypoxia:Takealittlesodiumlime(about5gram)and

4、1mouseintoawide-neckedbottle.Observeandrecordthegeneralcondition,respiratoryfrequencyanddepth,aswellasskinandmucosacolor.Sealthebottlewithrubberstopperandbegintimerecording.Repeattheobservationaboveatevery3minutesintervaluntilthedeathofthemice.Recordanyotherchangesatanytimeanditsfinalsur

5、vivalduration.Opentheabdomenofthemousecorpseinorderafterotherhypoxicexperimentsarefinished.Observethecolorofbloodandmakecomparison.（2）Hemichypoxia1)Hypoxiaofcarbonmonoxidepoisoning:Observethenormalmanifestationofthemouseplacedinawide-neckedbottle,whichisattachedtoasetofcarbonmonoxidegene

6、rationdevicepreparedbeforehand.Take3mlofformicacidsolutionintoatesttubebycalibrationpipette,followedbyslowadditionof2mlofconcentratedsulfuricacidsolutionalongtheinclinedtubewall.Sealthetubewithrubberstopperandobservethesameindexesasabove.Itshouldbenoticedthatoverheatingthetesttubebyalcoh

7、olburnerisdangerousduetopossibleliquidseethingfspeedingupgenerationofcarbonmonoxideisnecessary.(Furthermore,carbonmonoxidegeneratedtoorapidandtoo6muchwouldcausethemousedierapidlywithoutevidentchangeofbloodcolor.)2)Hypoxiaofsodiumnitritepoisoning:Take2micewithsamebodyweigh