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1、ChromatographiaDOI10.1007/s10337-013-2503-xORIGINALUltrasonic-AssistedNebulizationExtractionCoupledwithSPEandHPLCfortheDeterminationofTriterpenoidsinRootofEuphorbiapekinensisRupr.PeixuZhang•YongriJin•JiafengChen•HuaYao•HanqiZhang•AiminYu•XuwenLiReceived
2、:24February2013/Revised:17May2013/Accepted:12June2013ÓSpringer-VerlagBerlinHeidelberg2013AbstractInthiswork,anovelmethodbasedonultra-Introductionsonic-assistednebulizationextractioncoupledwithsolidphaseextraction(UANE-SPE)anddeterminedbyhighThedriedroot
3、ofEuphorbiapekinensisRupr.[1]isakindperformanceliquidchromatographywasdevelopedfortheofChinesemedicineandhasbeencommonlyusedforthedeterminationoftriterpenoidsinrootofEuphorbiapekin-treatmentofascites[2,3],edemaandcancer[46].EupholensisRupr.Theexperiment
4、alconditionsfortheUANE-andtirucallolareapairofenantiomersoftriterpenoidSPE,suchastypeofextractionsolvent,sampleamount,compoundsandmainlyactiveconstituentsinE.pekinensistypeandamountofsorbent,extractiontimeandvolumeofRupr.theelutionsolvent,wereexaminedan
5、doptimized.TheNumerousmethodshavebeendevelopedforthemethodwassuccessfullyappliedtodetermineeupholandextractionofactivecompoundsfrommedicinalplantssuchtirucallolinthedriedrootofE.pekinensisRupr.TheasSoxhletextraction(SE),Refluxextraction(RE)andrecoverieso
6、ftheanalyteswereintherangeofMacerationextraction(ME)etc.[79].However,thetra--189.1102.0%.Thelimitsofdetectionwere12lggforditionalextractionmethodsaretimeconsuming,solvent-1tirucalloland10lggforeuphol.Theextractionyieldsandsamplewasting.Inrecentyears,som
7、enewextractionobtainedbytheproposedmethodarehigherthanthosemethodshavebeenapplied,likematrixsolidphasedis-obtainedbytheconventionalextractionmethods,suchaspersion(MSPD),ultrasonic-assistedextraction(UAE),refluxandultrasonic-assistedextraction.Comparedwit
8、hmicrowave-assistedextraction(MAE),pressurizedliquidthetraditionalmethods,theproposedmethodcanreduceextraction(PLE)andsupercriticalfluidextraction(SFE)theconsumptionofthelabor,shortenthesampleprepara-[1016].Inthiswork,Ultrasonic-a
