In Vitro Evaluation of the Antibacterial Activities of MTAD in Combination with Nisin against Enterococcus faecalis

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1、BasicResearch—TechnologyInVitroEvaluationoftheAntibacterialActivitiesofMTADinCombinationwithNisinagainstEnterococcusfaecalis†‡ZhongchunTong,PhD,*LinZhou,PhD,JieLi,MS,WenkaiJiang,MS,*LifangMa,MS,*andLongxingNi,PhD*AbstractIntroduction:MTADisacommonintra

2、canalirrigant.herootcanalfloraplaysanimportantroleinpulpalandperiapicalinfections(1–3).AlthoughMTADinhibitsEnterococcusfaecalisinTTheprincipalobjectivesofrootcanaltreatmentaretoremovepathogensandtorootcanals,itsbactericidaleffectagainstE.faecalismaintai

3、ntherootcanalinadisinfectedstate.Enterococcusfaecalis,apathogenicremainstobeimproved.Nisin,anantibacterialpeptide,microorganism,isfrequentlyfoundinendodonticinfections,especiallyinsecondarypossessesastrongbactericidaleffect.Thestudyevalu-andpersistentr

4、ootcanalinfections(4).E.faecalisisabettersurvivorthanotheratedthesynergeticactionbetweenMTADandnisinbacteriafoundinrootcanalsandisresistanttovariousharshenvironmentssuchasagainstE.faecalis.Methods:Theminimalinhibitoryanalkalineenvironment(pH,9.6),biles

5、alt,starvation,andmanyantibacterialagentsconcentration(MIC)andminimalbactericidalconcentra-(4–7).Asaresult,itisdifficulttokillandcompletelyremoveE.faecalisfromthetion(MBC)wereusedtomeasuretheantibacterialactiv-infectedrootcanal.itiesofMTAD,MTAN(substitu

6、tionofdoxycyclinewithMTAD,anaqueoussolutioncomposedof3%doxycycline,4.25%citricacid,andnisin),andMTADN(nisinincombinationwithdoxycy-0.5%polysorbate80detergent,isoftenusedtoremoveendodonticsmearlayersandcline),respectively.Thesynergeticeffectbetweennisin

7、causesminimalerosivechangesonthedentinsurface(8,9).Itsantibacterialeffectanddoxycyclinewasevaluatedbyfractionalinhibitoryisattributedlargelytodoxycycline,aprimarycomponentthatisincludedtoinhibitconcentration(FIC)andtime-killingcurves.Further-bacteria.H

8、owever,resistancetodoxycycline,atetracycline,isnotuncommonamongmore,morphologicchangesinE.faecaliswerethebacteriaisolatedfromrootcanals,andtheantibiotichasanarrowspectrumobservedbyscanningelectronmicroscopyafterE.fae-(10).Chlorhexidineh