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1、BasicResearch—TechnologyInVitroEvaluationofMTADandNisininCombinationagainstCommonPathogensAssociatedwithRootCanalInfection†ZhongchunTong,PhD,*LinZhou,PhD,RongKuang,PhD,*HaipengLv,PhD,*TiejunQu,PhD,*andLongxingNi,PhD*AbstractIntroduction:ManypathogenicmicroorganismswereKeyWordsfoundinaninfectedroot
2、canal.TheobjectofthisstudyEnterococcusfaecalis,MTAD,MTADN,MTAN,nisinwastoevaluatetheeffectofMTADincombinationwithnisinonthepathogensassociatedwithrootcanalinfec-ntibacterialpeptide,anewclassoftherapeuticagents,isincreasinglyplayingantion.Methods:Thesurvivalratesof9pathogenicAimportantroleininhibit
3、ingpathogenicmicroorganismcausedbybacterialresis-bacteriaweredeterminedafter1-,5-,and10-minutetancetosomecommonantibiotics(1,2).Nisin,atypeAlantibiotic,hasbeenfoundtreatmentwithMTAD,MTAN(substitutionofdoxycy-toinhibitthegrowthandsporegerminationofmanygram-positivebacteriaandhasclinewithnisin),andM
4、TADN(nisinincombinationanantibacterialefficacyagainstmultidrug-resistantbacteriaincludingEnterococcuswithdoxycycline).ThesurvivalratesofEnterococcusfaecalis.Nisinis34-residueantibacterialpeptideproducedbyLactococcuslactisfaecalisinthestarvationphaseandpretreatmentalka-andhasexcellentpropertiessucha
5、slittletoxicity,heat-stable,odorless,colorless,taste-lizationaswellasinthenormalphysiologicalstateless,andactiveatlowpH(3).Nisinhaswidelybeenusedasapreservativeindairyprod-underMTAD,MTAN,andMTADNchallengefor1,5,uctsandisgenerallyrecognizedassafe(GRAS)bytheU.S.FoodandDrugand10minuteswereevaluatedan
6、dcompared.Further-Administration(4–6).Theadvantagesofnisinsuggestithasconsiderablepotentialmore,scanningelectronmicroscopywasusedtotobeusedasanantibacterialagenttoinhibitpathogensassociatedwithrootcanalobservethemorphologicmodificationofActino-infection.mycesnaeslundii,Lactobacillusparacasei,andThe
7、speciesofmicroflorainaninfectedrootcanalarediverse,includingPorphyr-PorphyromonasgingivalisafterMTADandomonasspp.,Fusobacteriumspp.,Peptostreptococcusspp.,Actinomycesspp.,MTADNtreatment.Results:L.fermenti,L.paraca