Roles of the catalytic subunit of

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1、FungalGeneticsandBiology47(2010)406–415ContentslistsavailableatScienceDirectFungalGeneticsandBiologyjournalhomepage:www.elsevier.com/locate/yfgbiRolesofthecatalyticsubunitofcAMP-dependentproteinkinaseAinvirulenceanddevelopmentofthesoilborneplantpathog

2、enVerticilliumdahliaeaa,*b,1bAlikiTzima,EpaminondasJ.Paplomatas,PayungsakRauyaree,SeogchanKangaLaboratoryofPlantPathology,AgriculturalUniversityofAthens,75IeraOdos,11855Athens,GreecebDepartmentofPlantPathology,ThePennsylvaniaStateUniversity,University

3、Park,PA16802,USAarticleinfoabstractArticlehistory:VerticilliumdahliaeisasoilbornefungusthatcausesvascularwiltdiseaseinabroadrangeofhostsandReceived28August2009survivesformanyyearsinthesoilintheformofmicrosclerotia.AlthoughtheroleofcAMP-dependentAccept

4、ed27January2010proteinkinaseA(PKA)hasbeenextensivelystudiedinfoliarpathogens,thereislimitedinformationAvailableonline6February2010aboutitsroleinsoilbornefungalpathogensthatinfectthroughtherootsystem.GenomedatabasesearchrevealedthepresenceoftwoPKAcatal

5、yticsubunitgenesinV.dahliae,namedVdPKAC1andVdPKAC2.Keywords:AphylogeneticanalysisshowedthatVdPKAC2groupswithfungalPKAcatalyticsubunitsthatappearSignalingtoplayaminorroleinPKAactivity.ThisgenewasexpressedconsiderablylowerthanthatofVdPKAC1.Pathogenicity

6、genesAlthoughdisruptionofVdPKAC1didnotaffecttheabilityofV.dahliaetoinfectthroughtherootsofWiltdiseasetomatoandeggplant,diseaseseveritywassignificantlyreduced.Sincepathogen-derivedethyleneispre-sumedtoplayamajorroleinsymptominductioninvascularwiltdiseas

7、es,ethylenegenerationwasmea-suredinfungalculture.ThemutantsdefectiveinVdPKAC1producedlessethylenethanthecorrespondingwildtypestrains,suggestingaregulatoryroleofPKAinethylenebiosynthesis.Growthratesofthesemutantsweresimilartothoseofwildtypestrains,whil

8、etherateofsporegerminationwasslightlyele-vatedandconidiaproductionwassignificantlyreduced.Whengrownonminimalmedia,themutantsshowedgreatermicrosclerotiaproductioncomparedwiththewildtypestrains.TheseresultssuggestmultiplerolesofVdPKAC1,includingv