Expression profiling of microRNAs in RAW264.7 cells treated

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1、JPeriodontalRes2012©2012JohnWiley&SonsA/SAllrightsreservedJOURNALOFPERIODONTALRESEARCHdoi:10.1111/jre.1201712T.Kagiya,S.Nakamura1ExpressionprofilingofDivisionofFunctionalMorphology,DepartmentofAnatomy,IwateMedicalUniversity,Iwate,2JapanandDivisionofMaxillofacialD

2、iagnosticmicroRNAsinRAW264.7andSurgicalSciences,DepartmentofOralandMaxillofacialOncology,KyushuUniversityGraduateSchoolofDentalScience,Fukuoka,cellstreatedwithaJapancombinationoftumornecrosisfactoralphaandRANKLduringosteoclastdifferentiationKagiyaT,NakamuraS.Exp

3、ressionprofilingofmicroRNAsinRAW264.7cellstreatedwithacombinationoftumornecrosisfactoralphaandRANKLduringosteoclastdifferentiation.JPeriodontRes2012;doi:10.1111/jre.12017.©2012JohnWiley&SonsA/SBackgroundandObjective:Tumornecrosisfactoralpha(TNF-a),acytokineinvolve

4、dinthepathogenesisofperiodontaldisease,inducesosteoclastdifferentia-tionandindirectlypromotesalveolarboneresorption.WeinvestigatedTNF-a-regulatedosteoclastdifferentiation,focusingonmicroRNAs.MicroRNAsaresmall,noncodingRNAsthatareinvolvedinvariousbiologicalprocesse

5、s,includingcellulardifferentiation,proliferationandapoptosis.AsidefrommiR-21,miR-155andmiR-223,theidentitiesofthemicroRNAsthatplayrolesinosteoclastdifferentiationareunknown.Notably,nopreviousstudieshavereportedtheexpres-sionprofilingofmicroRNAsduringTNF-a-regulated

6、osteoclastdifferentiation.MaterialandMethods:WeusedmicroarraystoscreenthelevelsofexpressionofmaturemicroRNAsinRAW264.7cellstreatedwithacombinationofTNF-aandRANKL,orRANKLalonefor0,24or82hduringosteoclastformation.Wevalidatedtheresultsofthemicroarrayanalysesthrough

7、quantitativeRT-PCRanalysesofrepre-sentativemicroRNAsinRAW264.7cellsandmurinebonemarrowmacrophages.Results:Duringosteoclastformation,theexpressionof44maturemicroRNAsdifferedbymorethantwofoldbetweenuntreatedcellsandcellstreatedwithacombinationofTNF-aandRANKL,andthe

8、expressionof52maturemicro-RNAsdiffereduponRANKLtreatment.AccordingtoquantitativeRT-PCRTadayoshiKagiya,DDS,DivisionofFunctionalanalyses,miR-378wasupregulatedandmiR-223w