effect+of+angelica+keiskei+chalcone+on+the+expression+of+apoptosis-regulating+proteins+of+mice+hepatocarcinoma+cells

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1、EfectofAngelicakeiskeichalconeontheexpofapoptosis--regulatingproteinsofmicehepatocarcinomacellsYangMeng，JinyiZhong，HeSunDepartmentofToxicology，MedicalCollegeofOjngdaoUniversity，Ojngdao266021，ChinaReceived：15January2011／Revised：20February2011／Accepted：17March

2、2011@HuazhongUniversityofScienceandTechnologyandSpringer-VerlagBerlinHeidelberg2011AbstraclObjective：TheaimofourstudywasloinvestigatetheefectofAngeficakeiskefchalcone(AC)ontheexpressionofCaspase．3andBaxinmiceheDal0carcinOmacells．Methods：Fiftymiceinoculatedhe

3、patocarcinoma22cellsweredividedintofivegroups，10micepergroup．Miceweregiven5，20，40mg／kgACdailybymouthinlow，middleandhighdosegroupsrespectively．Salineweregivenlolhelumorcentrelgroupbymouth．Twentymg／kgcytoxan(CTX)byinjectioneveryotherdayweregivenlolhepositivece

4、ntreIgroup．Tendayslater,alImiceweresacrificed．ThelevelsoflheCaspase一3andBaxproteinexpressionweremeasuredbyimmunohistochemistrymethodandlheproliferationactivityofhepatocarcinomacellswasdeterminedbyMTTassay．尺esults：TheexpressionIeveIofCaspase-3andBaxproteinint

5、umorcentreIgroupwere5．00％and4．68％．respectively,andthoseofthehigh．dosegroupwere38．52％and35．76％．ThedifferencesbetweentwogroupsweresignificanlfP<0．05)．ThecelproliferationactivityoflumorcentreIgroupandhigh-dosegroupwere1．135±0．032and0_716±0．018．Thediferencewassi

6、gnificanlfP<0．05)．Conclusion：ACcanincreaselheexpressionofCaspase-3andBaxprotein，andinhibittheproliferativeactivityofmicehepal0carcinOmacells．KeywordsAngelicakeiskeichalcone；hepatocarcinoma；Caspase-3：BaxAngelicakeiskeiisakindofparsleyfamilywildplantslaborator

7、y．Itspuritywas>90％whichwasdeterminatedwhichoriginatedfromJapan【lJ’whichcanimproveim—byUVspectrophotometry．C：halconestandardfpurity>munityandsleep[2J．Chalconeistheoneofthemaincom—99％1waspurchasedfromSigmaChemicalCorporation．ponentsinAngelicakeiskei．Researches

8、haveshownthatRPMI1640medium．ThiazolylBlueTetrazoliumBro—chalconehasremarkableanti．．tumorandanti．．oxidantac--midefMTT)andcalfseIxlmwerepurchasedfromGIBCOtivit)r[3]_Studieshaveshownthatapoptosisis