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1、APPLIEDANDENVIRONMENTALMICROBIOLOGY,Feb.2006,p.13461354Vol.72,No.20099-2240/06/$08.000doi:10.1128/AEM.72.2.13461354.2006Copyright©2006,AmericanSocietyforMicrobiology.AllRightsReserved.DifferentialEffectsofNitrogenousFertilizersonMethane-ConsumingMicrobesinRiceFieldandForestSoils1221SantoshR.Mohant
2、y,§PaulL.E.Bodelier,*VirgilioFloris,andRalfConrad1MaxPlanckInstituteforTerrestrialMicrobiology,DepartmentofBiogeochemistry,Karl-von-Frisch-Str.,35043Marburg,Germany,andNetherlandsInstituteofEcology(NIOO-KNAW),CentreforLimnology,DepartmentofMicrobialWetlandEcology,2Rijksstraatweg6,3631ACNieuwersluis
3、,TheNetherlandsReceived13September2005/Accepted18November2005Theimpactofenvironmentalperturbation(e.g.,nitrogenousfertilizers)onthedynamicsofmethanefluxesfromsoilsandwetlandsystemsispoorlyunderstood.Resultsoffertilizerstudiesareoftencontradictory,evenwithinsimilarecosystems.Inthepresentstudythehypot
4、hesisofwhetherthesecontradictoryresultsmaybeexplainedbythecompositionofthemethane-consumingmicrobialcommunityandhencewhethermethanotro-phicdiversityaffectsmethanefluxeswasinvestigated.Tothisend,ricefieldandforestsoilswereincubatedinmicrocosmsandsupplementedwithdifferentnitrogenousfertilizersandmethan
5、econcentrations.Bylabeling13themethanewithC,diversityandfunctioncouldbecoupledbyanalysesofphospholipid-derivedfattyacids(PLFA)extractedfromthesoilsatdifferenttimepointsduringincubation.Inbothricefieldandforestsoils,theactivityaswellasthegrowthrateofmethane-consumingbacteriawasaffecteddifferentially.
6、FortypeImethanotrophs,fertilizerapplicationstimulatedtheconsumptionofmethaneandthesubsequentgrowth,whiletypeIImethanotrophsweregenerallyinhibited.TerminalrestrictionfragmentlengthpolymorphismanalysesofthepmoAgenesupportedthePLFAresults.Multivariateanalysesofstable-isotope-probingPLFAprofilesindicate
7、dthatinforestandricefieldsoils,Methylocystis(typeII)specieswereaffectedbyfertilization.ThetypeImethanotrophsactiveinforestsoils(Methylomicrobium/Methylosarcinarelated)differedfromtheactivespeciesinricefieldso
