云南省几种作物与杂草上双生病毒与伴随的DNAβ的分子鉴定

《云南省几种作物与杂草上双生病毒与伴随的DNAβ的分子鉴定》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、浙江大学硕士学位论文AbstractMembersofthegenusBegomovirusinthefamilyGeminiviridaehavecircularsingle-strandedDNAgenomeencapsidatedintwinnedicosahedralparticles．BegomovimseshavecausedsignificantyieldlosstomanycropsandarebecomingaseriousthreattocropsinsouthChinasince1990s．Thecurre

2、ntstudywascarriedouttoidentifybegomovirusesinseveralcropsandweedsinYunnanprovince．FortybegomovimssampleswerecollectedfromNicotianatabacum，Lycopersiconesculentum，Malvastrumcoromandelianum，Solanumaculeatissimum，AgeratumconyzoidesandSiegesbeckiaoriental&plants．PCRdetect

3、ionusinguniversalprimerpairPMPBforbegomovimsesindicatedthatthesesampleswereinfectedbybegomovimses．Basedonthenucleotidesequencesofthe500bpfragmentsamplifiedbyPAandPB，the40isolatesconsistoffourmaintypes：Y231，Y238，Y240，Y241，Y244，Y248，Y261，Y262，Y263，Y264，Y265，Y266，Y268，Y

4、295，Y296，Y297，Y298，Y318，Y319，Y320，Y321，Y322，Y323andY324areisolatesofTomatoyellowleafcurlChinavirus(TYLCCNV)；Y288，Y289，Y291andY315areisolatesofTobaccocurlyshootvirus(Thcsv)；Y290，Y309，Y310，Y311，Y314，Y316andY317areisolatesofTobaccole矽curlYunnanvirus(TbLCYNV)；Y304，Y305，Y

5、306，Y307andY308areisolatesofMalvastrumyellowveinYunnanvirus(MYVYNV)．ThevirusisolateY322wascollectedfromSolanumaculeatissimumshowingleafcurlandenationsymptomsinYanshan，YunnanProvince．ThecompletenucleotidesequenceoftheDNA—AlikemoleculeofY322isdeterminedtobe2730nucleoti

6、des．Y322DNA—AismostcloselyrelatedtoisolatesofTYLCCNVwith88．3％～99．2％nucleotidesequenceidentities．whilelessthan79．6％nucleotidesequenceidentitiesarefoundincomparisonswithoth+rbegomoviruses．Therefore，Y322istaxonomicallyconsideredasanisolateofTYLCCNV．Furtherstudiesshowtha

7、tY322isassociatedwithaDNABmolecule．TheDNAl3ofY322contains1331nucleotideshavingthehighestnucleotidesequenceidentity(93．1％)withDNApassociatedwithTYLCCNV．whilelessthan55．4％nucleotidesequenceidentitiesarelV浙江大学硕士学位论文foundincomparisonswithotherDNA[jlmolecules．Thisisthefir

8、streportofTYLCCNVinfectingSolanumaculeatissimum．ThevirusisolatesY231andY244wereobtaJ【nedfromArt-cotianatabacumandY295fromLycopersic