促红细胞生成素减轻新生儿窒息后血清诱导HK-2细胞凋亡作用的胞内信号传导机制分析

《促红细胞生成素减轻新生儿窒息后血清诱导HK-2细胞凋亡作用的胞内信号传导机制分析》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、泸州医学院硕士研究生论文MechanismofIntracellularSignalTransductionofErythropoietinRelievetheApoptosisofHumanRenalTubularCellsInducedbyPostasphyxial·SerumofNeonatesAbstractMethods(1)Humanrenalproximaltubularcellline(IlK一2)wasusedastargetcell．(2)Thesertlmofneonatesinoneday

2、afterasphyxia(ApgarScoringlessthan4)，whoseconcentrationwere20％(volumefraction)，wereappliedasattackingfactor．(3)Theexperimentweredividedascontrolgroup，asphyxiagroup，groupofpretreatmentwithEPO(erythropoietin)，groupofpretreatmentwithEPOand5-HD(5一Hydroxydecanoica

3、cidsodiumsalt)．(4)Thefollowingindicatorsweredetected．Thechangesofmorphologywereobservedunderinvertedmicroscope；TheexpressionofOmi／HtrA2，caspase-3andXIAPincytoplastweredetectedbytheuseofimmunohistochemicalmethod；TheapoptosisrateofHK一2cellswasdetectedbyflowcyto

4、meter；Themitochondriamembranepotential(AVm)wasexamintedbyconfocalmicroscopy；ThetranspositionofOmi／HtrA2fromchondriosometoendochylemabyindirectimmunofluorescencedoublestainingwasobservedunderconfocalmicroscopy．(5)Thestatisticalsignificancebetweentreatmentswasa

5、nalyzedusingone—wayANOVA，wherep

6、quantityofHK一2cellwasnormal．Comparedwithcontrolgroup，thechangesinmorphologyofItK一2weremostseriousandobviousinasphyxiagroup，thecellsgrewslowly，themountdecreased，formofthecellschanged3泸州医学院硕十研究生论文fromtypicalmulty-angappiantooff-normalroundorellipse．Refractionra

7、tewasdecreased，andcontourenhanced．Thevacuolus，lipiddropletandgranulationappearedinthekytoplasm．Therewasmuchcelldebrisinaccrescentintercellularspace．Butcomparedwithasphyxiagroup，thechangesinmorphologyofHK一2wereobviouslyimprovedinthegroupsofpretreatmentwithEPO．

8、However，thoseprotectiveeffectscanbeeliminatedby5-HD．(2)Comparedwithcontrolsgroup(22．75+4．03，32．13+5．46，34．88+4．39)，theexpressionofOmi／HtrA2(51．25+3．69)andcaspase-3(62．88+4．70)weresignific