Bcl-2和Bax在DEN诱发大鼠肝癌细胞上的表达

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1、THEEXPRESSloNoFBcl．2ANDBaxoNDEⅣINDUCEDLIVERCANCERoFRATPostgmduate：LiuHongfhSp∞iamy：HumanA皿atomyandEmbryolo斟Superyisor：Pro￡WangZengxianABSTRACTobjective11leliverc锄cermodelw嬲iIlduced诹mdieⅡlylIlitros锄1ine(DEN)toobserye廿leregularit)rofliVerceUapoptosisandexpres

2、sionof印optosisrel劬edgeneBcl-2andBa)【intheilldllcedc锄cer，andtoexplore廿lerelationshipbe觚een印optosisandtlledevelopmentof1iverc锄c％acc嘲ulatingeXp鲥mental胁underlyingⅡlemech趾ismoflivercancer．methodsEighty—eightm：tSwereused，72ra：tS私expe胁entm粤-oupi11ducedbydIi玎kiIlgw

3、atercontaillingDEN，8rats硒control留．oup．触erlap锄tomy，也efo皿，color，tex眦ofnleliver锄dm删i嬲isiJlbotllcontrol觚de)【pe血entalgroupswereobservedandrecorded．Themetastatictumor觚dⅡlelivertisSuewefesectionedandStainedwithhematoxylin—eosins诎血gt0denlonS眦mech锄cteristiciIlmo叩hol

4、ogicalchaIlges，thehochst33342fluorescem幽gw鹤印pliedt0show勰dcounttllera：teofapoptoticcell，andtlleinshhybridizationtechIliq鹏Ⅵ粥邯edt0detcctnleBcl一2mRNAandBa)【mRNAexpression．I℃sults1．Accordir培t0thepatholo百calch趴geofliverceUs，meprocessofcarciIlogenesisc孤bedividedin

5、_t0t11reeperiods：h印atictoxiclesion，hepaticproliferation／cimlosis觚dh印aticcarcillog即esis．(1)IIlh印撕ctoxiclesionp喇od，noobviollSa_bno册alwasobservedontlleliversul．fke．Under廿lemicroscopyⅡlellepaticlobulew嬲iIlitSin_te面t)r，but谢tlldifhJseedem％如c甜necrosisaccompaIlied诵

6、tllinn砌at0巧cellinfil蹴ioIl，somecellsiIlnlelobllles_howedeos劬pllilicdegene枷on．Theh印aticcenregemr撕on觚dfibroustissueprolifemtionwerealsodemoIls咖ted．(2)Inh印aticpmlifcmtioIl／ciIrhosisperio也meliverswellcd如mleranditssumICebec锄erou曲砒hdif觚iIlgfoci，grey觚dnearlyc硫ul鸡恤：

7、liVcrc衙hosisstaned．Undern圮嘶croscopy，廿le1iVercells、析thless铆iap∞life删ill_t0nodllles，锄deosinopmlicdegenemtionandhyaliIle3prolif．e枷onwereobserved．(3)Inhep撕ccarcillog∞esisp耐od’theliV盯w弱CoVeredbygrcynodulesinw撕。璐s妇：s，锄dtheheomo盯ha西cspotS锄dnecrosis袱肥aJs0Se饥onme删io

8、n．Underthemicr0sClopy，t11eliVerShowcdallsig璐ofhepaticCenc锄cer：cells锄加．gcdincord粕dl啪pmski11filtratedi11：t0sun．0衄diIlgliVertis跚e，锄dV撕ollsdegreesoff-att)rdegen训。玛regiIlmheomom嘤e觚dnecrosiswerea1S0detect甜．P