资源描述：
《13 Surface Trafficking of Membrane Proteins at Excitatory and Inhibitory Synapses 》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、SurfaceTraffickingofMembraneProteinsatExcitatoryandInhibitorySynapses12DanielChoquetandAntoineTriller1UMR5091CNRS,UniversitédeBordeaux2,PhysiologieCellulairedelaSynapse,InstitutFrançoisMagendierueCamilleSaintSaëns33077BordeauxCedex,France,dchoquet@u-bordeaux2.fr2InsermUR497,EcoleNormaleSupé
2、rieure,BiologieCellulairedelaSynapseN&P,46,rued'Ulm75005Paris,France,triller@biologie.ens.fr1IntroductionUntilrecently,synapseorganizationwasseenasratherstable,beingmodifiedonlyduringplasticprocessesovertimecoursesrangingfromminutestohours.Bulkap-proaches,i.e.probinglargenumbers(fiftytofewh
3、undreds)ofmoleculessimultane-ouslyhaspermittedtheobservationofdynamicreorganizationofsynapticcompo-nents,andthishaschallengedthenotionthatsynapsesarestableentities.However,theseapproachesdidnotallowthebehaviorofindividualmoleculestobefollowed.Thefirstsinglemoleculetechniquetobeusedinsynapti
4、cbiologywaspatch-clamprecordingsmeasuringtheelectrophysiologicalpropertiesofindividualionchannels.Thisapproach,thoughpowerful,doesnotallowforadeterminationofthespatialdistributionofagivenmoleculeovertime.Astraffickingofmolecularcomponentshasemergedasamajorpathwayintheregulationofsynapsefunc
5、tion,thefieldwassearchingformeanstoinvestigatethemicroscopicbehaviorofmoleculeswithhighspatio-temporalresolution.Oncetheconceptwasestablishedthatsynapticmoleculesareinmovement,itwasimplicitthattheyareinreversibleinteractionsinashorttimescale(i.e.receptorscaffoldinteractions).Therecentdevelo
6、pmentsinsinglemoleculeimagingtechnologiesarenowlead-ingtowideapplicationsincellularbiologyallowingfortheunravelingofnewmechanismsrelatedtomolecularmovements.Theseadvancesweremadepossiblebyacombinationofimprovementsinopticaldetectorscombinedwithnewmolecu-lartaggingstrategiesandtheadventofbio
7、compatiblenanotechnologicaltools.Singlemoleculeimaginghasbeencriticalforsynapticbiologysincemoleculestrafficbetweenmicrodomainswithspecificmolecularcomposition.Inrecentyears,fluorescencerecoveryafterphotobleaching(FRAP,e.g.(3,143))andsingleparticletracki
