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1、生物工程学报ChinJBiotech2008,August25;24(8):1381-1386journals.im.ac.cnChineseJournalofBiotechnologyISSN1000-3061cjb@im.ac.cn©2008InstituteofMicrobiology,CAS&CSM,Allrightsreserved研究报告壳聚糖基角膜细胞载体的制备及其细胞相容性高兴爽,刘万顺,韩宝芹,位晓娟中国海洋大学海洋生命学院,青岛266003摘要:为探讨羟丙基壳聚糖基共混膜作为组织工程技
2、术中角膜细胞培养载体的可行性,分别制备了羟丙基壳聚糖/硫酸软骨素、羟丙基壳聚糖/明胶/硫酸软骨素以及羟丙基壳聚糖/氧化透明质酸/硫酸软骨素三种共混膜。测定其透光率、含水量和蛋白吸附性能;在共混膜上培养兔角膜上皮细胞,通过观察角膜上皮细胞在不同载体膜上的生长状态、贴附情况,测定细胞活性以及上清液中乳酸脱氢酶的活性,研究三种壳聚糖基载体膜片与角膜上皮细胞的相容性。膜片理化性质测定结果表明三种共混膜片具有良好的透明度,适宜的含水量和较强的蛋白吸附性能;细胞相容性实验结果表明羟丙基壳聚糖/明胶/硫酸软骨素共混膜对
3、细胞的损伤最小,有利于细胞在膜上的贴附和生长,表现出良好的细胞相容性,有望作为角膜细胞载体体外构建组织工程化角膜。关键词:壳聚糖,载体,角膜上皮细胞,细胞相容性PreparationandCytocompatibilityofChitosan-basedCarriersofCornealCellsXingshuangGao,WanshunLiu,BaoqinHan,andXiaojuanWeiCollegeofMarineLifeSciences,OceanUniversityofChina,Qingda
4、o266003,ChinaAbstract:Tostudythepossibilityofusinghydroxypropylchitosan-basedblendmembranesascarriersofcornealcellsintissueengineering,wepreparedthreekindsofblendmembraneslabeledhydroxypropylchitosan/chondroitinsulfate,hydroxypropylchitosan/gelatin/chondr
5、oitinsulfateandhydroxypropylchitosan/oxidizedhyaluronicacid/chondroitinsulfate.Thetransparency,watercontentandabilityofproteinadsorptionoftheblendmembranesweremeasured.Toevaluatethecytocompatibilityoftheblendmembraneswithcornealepithelialcells,rabbitcorne
6、alepithelialcellswereculturedonthesurfaceofthecarriermembranes.Themorphologicalcharacteristics,celladhesion,cellproliferationandtheactivityoflactatedehydrogenase(LDH)inthemediawereinvestigated.Threekindsofblendmembraneshadgoodopticaltransmittance,suitable
7、watercontentandabilityofproteinadsorption.Theresultsshowedthatthelessinjurywasmadetocornealepithelialcellsbythehydroxypropylchitosan/gelatin/chondroitinsulfateblendmembranethantheothers.Thiskindofmembranewasfavorofthegrowthandadhesionofcornealepithelialce
8、lls.Thehydroxypropylchitosan/gelatin/chondroitinsulfateblendmembraneisapromisingcarrierofcornealcellsandcanbeusedinreconstructionoftissueengineeredcornea.Keywords:chitosan,carrier,cornealepithelialcell,cytocompatibi
