新生牛皮胶原蛋白海绵的制备及其体外细胞相容性

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1、中国组织工程研究与临床康复与，2誊第42008—10—07出版如“rnafCffn把afR妇6咖e订ss“e西增nerfReeⅡrc^0c幻r7，2008f．，2，№．，{l：‰

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7、，pepsin+g1acia1aceticacid，sa1ting—out，dia1ysisandfreezedrying．Theobtainedco1lagenspongewasinocu1ated73o07nGaI1suwith矗broblastsuspension，whichweredividedintocollagenspongegroup，negatiVecontrolgroup(saline)andpositiVePIovince．0血acontrolgroup(rubberbungleachingliquor)．MAIN0UTC0MEMEASURES

8、：Invertedphasec0ntrastmicroscopeandJVCdigicalcamerasystemwereusedt00bserveMaong-Ⅻ☆．StLldV_ngflnrdoc啪te，thecelIm0rphologyandgmwth．AcfidjneorangedyeingwasusedtoobseⅣethepro1iferationofcelljncol1agenspongeatAssoc1a坨invesdga幻L20and35daysOfculture．HematOxylin—e0sinstainingwasusedtOObservet

9、hegmwthOfcellsincOllagenspOngeat65daysKbo0fOfculture．Bi0en曲1ee血20fStafcRESULTS：n1eceIlsofpositivecontrl01groupwerenOtadhesiveanda1ldiedthI_eedays1ater．Those0fcol1agenspOngeEmcale0mc【ee．LifeScienceandgr0upandnegatiVec0ntrOlgrOupwerenOn11alandadhesiVe．WimmeprOlOngOfcu1turetime，thespOnge

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