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1、·648·中医杂志2010年7月第51卷第7期JournalofTraditionalChineseMedicine,2010,Vol.51,No.7补肺化痰方对肺虚痰阻证模型大鼠++Na-K-ATP酶活性的影响李小兵沈丽萍刘小虹王丽新吴启端江凌燕蔡彦(广州中医药大学第一附属医院实验中心,广东省广州市机场路16号,510405)[摘要]目的探讨肺虚痰阻证模型大鼠Na+-K+-ATP酶活性变化及补肺化痰方的干预效果。方法30只SD大鼠随机分为正常组、模型组和治疗组各10只,采用冷风刺激加烟熏法致大鼠肺虚痰阻证模型,治疗组给予补肺
2、化痰方,正常组和模型组给予等++体积生理盐水,共14天。观察各组大鼠一般活动情况、浓氨水刺激后3min内咳嗽次数、大鼠肺组织病理改变及Na-K-ATP酶活性变化。结果用浓氨水刺激诱发咳嗽后,3min内模型组及治疗组咳嗽次数较正常组增加(P<0.05);治疗组大鼠病理切片示气管、支气管杯状细胞增生,腺体肥大、增生均较模型组减轻;模型组和治疗组的Na+-K+-ATP酶活性均低于正常组(P<0.05),但治疗++++组明显高于模型组(P<0.05)。结论补肺化痰方可上调肺虚痰阻证模型大鼠Na-K-ATP酶活性,肺泡膜上Na-K-AT
3、P酶的活性降低可能是产生肺虚痰阻证的机制之一。[关键词]肺虚痰阻;Na+-K+-ATP酶;补肺化痰方++EffectofBufeiHuatanFormulaontheActivityofNa-K-ATPaseinRatModelswithPhlegmRetentionduetoLungDeficiencyLIXiaobing,SHENLiping,LIUXiaohong,WANGLixin,WUQiduan,JIANGYong,LINGYan,CAIYan(TheFirstAffiliatedHospitalofGuangzh
4、ouUniversityofChineseMedicine,Guangzhou510405)ABSTRACTObjectiveTostudytheeffectofBufeiHuatanFormula(Herbalformulaforreinforcingthelungtodissolvephlegm)on++thechangesofactivityofNa-K-ATPaseoftheratmodelswithphlegmretentionduetolungdeficiency.MethodsThirtySDratswerera
5、ndomizedintonormalgroup,modelgroupandtreatmentgroupwith10ineach.Themodelsoflungdeficiencywithphlegmre-tentionweremadewithcoldwindandsmoking.ThetreatmentgroupreceivedBufeiHuatanFormula,thenormalgroupandmodelgroupweregivennormalsalineofequal-volumefor14days.Generalact
6、ivities,timesofcoughinducedbythestimulationofconcen-++tratedammoniawithin3minutes,pathologicalchangesinthelungtissues,andchangesofactivityofNa-K-ATPasewereobserved.ResultsThetimesofcoughofthemodelandtreatmentgroupswereincreasedcomparedtothatofthenormalgroup(P<0.05).
7、Thepathologicalsectionofthetreatmentgroupshowedthatthegobletcellhyperplasiaandglandularhypertrophyoftracheaandbronchi++weremilderthanthoseofthemodelgroup.TheactivityofNa-K-ATPaseofthemodelandtreatmentgroupwaslowerthanthatofthenormalgroup(P<0.05),butthetreatmentgroup
8、wassignificantlyhigherthanthemodelgroup(P<0.05).ConclusionBufeiHuatanFormulaisabletoup-regulatetheactivityofNa+-K+-ATPaseofthemodelratswit
