资源描述：
《理化法快速鉴别周围神经束性质》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、·80·ChineseJournalofReparativeandReconstructiveSurgery,January2008,Vol.22,No.1理化法快速鉴别周围神经束性质＃＃王虎路来金刘东昕【摘要】目的探索一种快速鉴别感觉与运动神经束的方法。方法纯种Wistar大鼠30只，处死后取整条脊柱。取脊神经节及背根纤维、腹根纤维及坐骨神经，采用免疫印迹实验观察AnnexinV和Agrin特异性。其中15只大鼠作为实验组，取脊神经前根、后根、坐骨神经及其肌支、皮支行免疫组织化学染色；余15只用PB
2、S代替一抗作为对照组。12月龄健康新西兰大白兔16只，采用喇曼光谱法对不同性质的神经束进行研究。结果AnnexinV和Agrin分别是感-1觉神经与运动神经中的特异物质。运动神经与感觉神经的喇曼光谱在1088、1276、1439、1579及1659cm等附近的喇-1-1曼振动峰相对强度存在明显差异，1276cm处的峰值与1439cm处的峰值比I1276/I1439分别为0.95±0.06和1.17±0.08，提示运动神经与感觉神经间的微观结构差异（P<0.05）。结论显微激光喇曼光谱法是一种鉴别神经束
3、性质的有效的物理学方法，优于免疫组织化学法，具有临床应用的可行性。【关键词】神经束免疫组织化学法显微喇曼光谱鉴别中图分类号： R338R446.1文献标志码：AIDENTIFICATIONOFPROPERTYOFNERVEFASCICLESBYPHYSICALANDHISTOCHEMISTRYMETHODS/WANGHu,LULaijin,LIUDongxin.DepartmentofHandSurgery,theFirstHospital,JilinUniversity,ChangchunJilin
4、,130021,P.R.China.Correspondingauthor:LULaijin,E-mail:wanghu101@126.com【Abstract】Objective Toexploreamethodtoidentifythesensoryandmotorfasciclesinperipheralnervetrunk. Methods ThirtyWistarratswereselectedtoobtainwholespine.Thespinalganglion,itsdorsalroo
5、tandventralroot,andsciaticnervewereharvested,AnnexinVandAgrinspecificitieswereobservedwithWesternblot.Intheexperimentalgroup,anteriorbranchandposteriorbranchofspinalnerve,sciaticnerve,anditsmuscularbranchandcutaneousbranchwerehar-vestedfrom15ratstomaket
6、heobservationofimmunohistochemistry.Intheother15rats,firstantibodywasreplacedbyPBSascontrolgroup.DifferentnervefascicleswerestudiedwithMicroRamanscatteringtechniquein1612-month-oldNewZealandrabbits. Results TheAnnexinVandAgrinwerespecialsubstancesofsens
7、oryandmotornervesrespectivelyandcanactasspecificantigensforidentifyingdifferentnervefascicles.Thereweresignificantdifferencesintheintensityandbreadthofthe-1peakofthespectralpropertiesbetweenmotorandsensoryfasciclesatfrequenciesof1088,1276,1439,1579and16
8、59cm.-1Thepeakintensityratiosof1276to1439cmwere0.95±0.06inmotornervefasciclesand1.17±0.08insensoryfascicles,show-ingsignificantdifferences(P<0.05). Conclusion TheMicroRamanspectraismoreeffectivethanimmunohistochemistryinidentifyi
