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1、LC–Tandem-MSValidationfortheQuantitativeAnalysisofLevonorgestrelinHumanPlasma2008,68,7077121,21,&1111,&FeiLiu,YuXu,AixiangLiu,FangXu,WanqunHu,QingxiangGuo1DepartmentofChemistry,UniversityofScienceandTechnologyofChina,230026Hefei,China;E-Mail:xyu@ustc.edu.cn
2、;qxguo@ustc.edu.cn2FoodandChemicalProductsTestingSection,FujianProvincialCentralInspectionInstitute,350002Fuzhou,ChinaReceived:8April2008/Revised:5July2008/Accepted:7July2008Onlinepublication:19August2008[3–6].Furthermore,duetothestrongsideeffects,thedetecti
3、onoflevonorge-AbstractstrelinplasmaisimportanttoprovideinformationforpharmacokineticorInthisstudy,arapid,sensitiveandreproducibleliquidchromatographytandemmassbioavailabilitystudies.spectrometrymethodforthedeterminationoflevonorgestrelinhumanplasma,wasdevel
4、-Therehavebeenseveralreportsforopedandvalidated.Withastructuralanaloguenorethindroneastheinternalstandard,thedeterminationoflevonorgestrel,levonorgestrelwasextractedfromplasmausingethylacetate.Theorganiclayerwasincludingtheuseofradioimmunoassayevaporatedtod
5、rynessandtheresiduewasreconstitutedinmobilephase.Analiquotof[7],spectrophotometry[8,9],voltam-20lLwaschromatographicallyanalyzedonaPhenomenexLunaC18columnwithwatermetry[10]orthin-layerchromatographyandacetonitrileasthemobilephase.Selectedreactionmonitoringw
6、asspecificformass[11,12],butthemethodswerenotsen-detectionemployingpositiveelectrosprayionization.Thecalibrationstandardswerelinearsitiveenoughforpharmacokineticanal--1ysisoflowdosesoflevonorgestrel.Wangovertheconcentrationrange0.62540ngmL.Theintra-andinter-
7、dayprecisionovertheentireconcentrationrangewerelessthan8.16%.Themethodwasfoundtobesuitableforetal.[13]describedaliquidchromato-applicationtoapharmacokineticstudyafteroraladministrationof1.5mglevonorgestrelgraphy–tandemmassspectrometrytabletto20healthyfemale
8、volunteers.(LC–MS–MS)methodforthedetermi-nationoflevonorgestrelinhumanserum,withalowerlimitofquantifica--1tion(LLOQ)of5ngmL.MatejicekKeywordsetal.[14]employedliquidchromatog-raphy-ion-trapmassspectromet
