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1、ClinicalChemistry49:121976–1980(2003)MolecularDiagnosticsandGeneticsQuantitativeAnalysisandPrognosticImplicationofSARSCoronavirusRNAinthePlasmaandSerumofPatientswithSevereAcuteRespiratorySyndrome1312EndersK.O.Ng,DavidS.Hui,K.C.AllenChan,EmilyC.W.Hung,13311RossaW.K.Chiu,Nelso
2、nLee,AlanWu,StephenS.C.Chim,YuK.Tong,341*JosephJ.Y.Sung,JohnS.Tam,andY.M.DennisLoBackground:TheavailabilityofanearlydiagnostictoolSARS-CoVconcentrationsinpatientswhorequiredandforsevereacuterespiratorysyndrome(SARS)woulddidnotrequireintensivecareunitadmissionduringthehavemaj
3、orpublichealthimplications.Weinvestigatedcourseofhospitalizationwere5800and140copies/mL,whethertheSARScoronavirus(SARS-CoV)canberespectively(Mann–Whitneytest,P<0.005).ThesedatadetectedinserumandplasmasamplesduringtheearlywereconfirmedbytheRT-PCRsystemforthenucleo-stagesofSAR
4、Sandstudiedthepotentialprognosticcapsidregion,whichshowedanevenhigherdetectionimplicationsofsuchanapproach.rateof87%.Thecorrelationbetweentheresultsob-Methods:Wedevelopedtworeal-timequantitativere-tainedbythetwoRT-PCRsystemswashigh(Pearsonversetranscription-PCR(RT-PCR)assays
5、,oneforthecorrelationanalysis,r0.998;P<0.001).polymeraseandtheotherforthenucleocapsidregionofConclusion:Plasma/serumSARS-CoVquantificationthevirusgenome,formeasuringtheconcentrationofrepresentsapotentiallyusefulearlydiagnosticandSARS-CoVRNAinserum/plasmasamplesfromSARSprogn
6、ostictoolforSARS.patients.Plasmasampleswereobtainedfrom12con-©2003AmericanAssociationforClinicalChemistryfirmedSARSpatientsonthedayofhospitaladmission,5aswellasondays7and14afterfeveronset.SerumSevereacuterespiratorysyndrome(SARS)isarecentlysampleswerealsoobtainedfrom23confir
7、medSARSemergedinfectiousdiseasecausedbyanovelcoronavi-patientsonthedayofhospitaladmission,11ofwhomrus,theSARScoronavirus(SARS-CoV)(1–4).Todate,subsequentlyrequiredintensivecare.ViralRNAwasmoleculartestingforSARShasfocusedmainlyonreverseextractedfromtheplasma/serumsamples.The
8、extractedtranscription-PCR(RT-PCR)analysisofnasopharyngealRNAwassubjectedto
