Simultaneous chemical fingerprint and quantitative analysis

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1、AnalBioanalChem(2010)396:3087–3095DOI10.1007/s00216-010-3536-8ORIGINALPAPERSimultaneouschemicalfingerprintandquantitativeanalysisofGinkgobilobaextractbyHPLC–DADDaoquanTang&DongzhiYang&AnbangTang&YuanyuanGao&XianglanJiang&JieMou&XiaoxingYinReceived:17December2009/Revi

2、sed:26January2010/Accepted:30January2010/Publishedonline:27February2010#Springer-Verlag2010AbstractAreverse-phaseliquidchromatographymethodtionofchromatographicfingerprintandquantificationwithdiodearraydetectionwasdevelopedtoevaluatetheanalysiscouldbereadilyutilizeda

3、saqualitycontrolqualityofGinkgobilobaextractthroughestablishingmethodforG.bilobaextractanditsrelatedtraditionalchromatographicfingerprintandsimultaneousdetermina-Chinesemedicinalpreparations.tionofeightflavonoidcompounds,namelyrutin,myricetin,quercitrin,quercetin,lut

4、eolin,kaempferol,apigenin,andKeywordsColumnliquidchromatography.Fingerprintisorhamnetin.Thechromatographicseparationwasper-analysis.Flavonoidscompounds.GinkgobilobaextractformedonanAgilentSB-C18column(250×4.6mm,5.0µm)withagradientelutionprogramusingamixtureofmethanol

5、and0.1%formicacid(v/v)asmobilephaseIntroductionwithin55minat360-nmwavelength.ThecorrelationcoefficientsofsimilarityfordifferentbatchesofG.bilobaHerbalmedicineshavebeenwidelyusedforhealthneedsextractfromthesamemanufacturerandG.bilobaextractovermanycenturiesandbecomemo

6、reandmorepopularfromdifferentmanufacturersweredeterminedfromtheLCworldwideduringthelastdecade[1].However,duetothefingerprints,andtheysharedaclosesimilarity.Theeightfactthatinthoseherbstheremaybehundredsofcomplex2flavonoidcompoundsshowedgoodregression(R>0.9995)activec

7、omponentsofwhichwehavelimitedknowledge,itwithintestranges,andtherecoveryofthemethodwasinisalmostimpossibletoidentifyallthesesubstancesandtotherangeof94.1–101.4%.Inaddition,thecontentofthosecarryonquantitativeanalysis.AccordingtothetheoryofeightflavonoidcompoundsinG.b

8、ilobaextractpreparedbytraditionalChinesemedicine(TCM),thetherapeuticactionsdifferentmanufacturersofChinawasdeterminedtoofherbalmedi