a quantitative analysis of hydrogen production efficiency of

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1、internationaljournalofhydrogenenergy35(2010)1128–1137Availableatwww.sciencedirect.comjournalhomepage:www.elsevier.com/locate/heAquantitativeanalysisofhydrogenproductionefficiencyofTtheextremethermophileCaldicellulosiruptorowensensisOLAhmadA.Zeidan*,EdW.J.vanNielDepar

2、tmentofAppliedMicrobiology,LundUniversity,P.O.Box124,SE-22100Lund,SwedenarticleinfoabstractTArticlehistory:CaldicellulosiruptorowensensisstrainOL(DSM13100)isanobligatelyanaerobic,extremeReceived20October2009thermophilicbacteriumthatiscapableofutilizingabroadrangeofc

3、arbohydratesandReceivedinrevisedformproducingH2asametabolicby-product.TheperformanceofC.owensensisonglucoseand18November2009xylosewasanalyzedinlab-scalebioreactorstoassessitspotentialuseinbiohydrogenAccepted21November2009production.Acetate,H2,andCO2werethemainendpro

4、ductsduringexponentialgrowthofAvailableonline16December2009theorganismoneithersugar.LactateproductionwastriggeredduringthetransitionintothestationaryphaseandwasassociatedwithanincreaseinthelevelsofspecificL-lactateKeywords:dehydrogenaseactivity.Inaddition,minoramount

5、sofethanolandpropionatecouldbeBiohydrogendetected.H2andacetateyieldswereloweronxylosethanonglucose,markinganoppositeExtremethermophiletrendtobiomassandlactateyields.TheinfluenceofelevatedH2partialpressureonCaldicellulosiruptorowensensisproductdistributionwasmoredrama

6、ticinxylose-fermentingcultures.ReplacementofBatchmodeyeastextractinthemediumwithastandardvitaminssolutionimprovedH2yieldonbothH2partialpressuresugars,whereitreached100%ofthetheoreticalmaximum,i.e.4molpermolhexose,onLactateformationglucose.Byusingthedefinedmedium,both

7、themaximumspecificgrowthrateandtheDefinedmediummaximumvolumetricH2productionrateofC.owensensisincreasedsignificantlyonglucoseandalmostdoubledonxylose.Screeningothersugarsbesidesglucoseandxyloserevealedaclearsugar-dependentproduct-distributionpatternandadirectcorrelatio

8、nbetweenbiomassandlactateyields,whichmightbeexplainedconsideringenergymetabolismofthecells.Theorganismisproposedasanewcandidateforbiohydro